摘要
本实验复合蛋白酶酶解处理豆粕制备大豆小肽,酶解产物经SephadexG-25凝胶过滤层析,并对其各组分分子量分布及其促进益生菌发酵作用进行了研究,结果显示,大豆蛋白酶解物经SephadexG-25分离后得到了六个肽片段,分子量大部分在1 000 D以下,体外单细菌培养方法测定了大豆小肽对动物肠道常见益生菌的4个菌株(包括粪链球菌、蜡样芽胞杆菌、枯草芽胞杆菌、酵母菌)增殖的影响,结果表明SBP(Soybeanpeptides)能促进这4株细菌的增殖。添加大豆小肽后益生菌Q68与致病菌混合培养实验中,40 h内可以彻底清除金黄色葡萄球菌,48 h内可以完全消灭大肠埃希菌,大豆肽可以促进有益菌的增殖,抑制有害菌的生长。
Soybean meals were digested by soybean protease.Then soybean peptides（SBP） were prepared by gel filtration chromatography using Sephadex G-25.And the function of SBP to promote the growth of probiotics was studied.Results showed that the molecular weights of the 6 separated fractions were under 1000D.SBP significantily promoted the growth of common probiotics（Streptococcus faecium cernelle,Cerea Spore Bacilli,Bacillu s subtilis and yeast fungus） in vitro.S.aureus was cleared away within 40 hours and E.coli disappeared within 48 hours in a mixed culture of SBP with one of the 4 probiotics.
出处
《中国微生态学杂志》
CAS
CSCD
2012年第4期311-313,317,共4页
Chinese Journal of Microecology
关键词
大豆小肽
益生菌
促生长
发酵
Soybean peptides
Probiotics
Growth promoting
Fermentation
