摘要
采用正交试验等设计,系统开展了光皮桦组织培养高效再生体系研究。结果表明:光皮桦茎段外植体最佳诱导培养基及激素组合为MS+0.50mg.L-1 6-BA+0.10mg.L-1 TDZ+30mg.L-1蔗糖+5.50g.L-1琼脂,丛生芽最佳生根培养基为1/2MS+1mg.L-1 IBA+20g.L-1蔗糖+5.50g.L-1琼脂;丛生芽在最佳生根培养基上培养15d后获健壮生根苗,移栽成活率达90%。该实验结果为光皮桦的优良品种快繁以及遗传转化体系建立奠定了良好的基础。
With orthogonal experiment design,a systematic research of tissue culture and efficient regeneration system of Betula luminifer was carried out.The appropriate medium for differentiation of stem explants was MS＋0.50 mg·L-1 6-BA＋0.10 mg·L-1 TDZ＋30 g·L-1 sugar＋5.50 g·L-1 agar,and the suitable rooting medium was 1/2MS＋1 mg·L-1 IBA＋20 g·L-1 sugar＋5.50 g·L-1 agar.The survival rate reached to 90%.These results could lay a good foundation for multipropagation and genetic transformation of B.luminifera.
出处
《西北植物学报》
CAS
CSCD
北大核心
2012年第3期604-610,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金项目(30600484)
浙江省重大科技专项(2008C02004-1)
浙江省林业科技重点项目(2006SY01)
关键词
光皮桦
组织培养
高效再生
茎段
Betula luminifera
tissue culture
efficient regeneration
stem
