摘要
目的探讨右美托咪定(Dex)对烫伤大鼠心肌损伤的影响。方法 SPF级雄性SD大鼠60只,体重200～250 g,随机分为4组(n=15):假烫伤组(S组)、模型组(C组)、咪达唑仑组(M组)和Dex组(D组)。制作30%体表面积(TBSA)Ⅲ度烫伤模型,烫伤后S组和C组静脉输注生理盐水,M组经颈静脉导管持续输注咪达唑仑0.6 mg/(kg.h),D组静脉输注Dex 5μg/(kg.h),至烫伤后6 h取腹主动脉血,测定血液流变学指标、血浆肿瘤坏死因子-α(TNF-α)、心肌肌钙蛋白I(cTnI)水平,并观察心肌组织形态学改变情况等。结果与S组比较,C组、M组和D组全血黏度(ηb)、红细胞比容(Hct)以及cTnI含量明显升高(P<0.05);与C组比较,M组和D组血ηb、Hct以及cTnI含量降低(P<0.05);与M组比较,D组血ηb和cTnI含量明显降低(P<0.05);与S组比较,C组、M组和D组血浆丙二醛(MDA)以及TNF-α含量显著增高(P<0.01),超氧化物歧化酶(SOD)活性降低(P<0.01);与C组比较,D组血浆MDA以及TNF-α含量显著降低(P<0.01),SOD活性增加(P<0.01);与M组比较,D组血浆MDA和TNF-α含量降低(P<0.05)。结论 Dex可以有效改善大鼠严重烫伤后的微循环和心肌损伤。
Objective To investigate the effect of dexmedetomidine on burn-induced myocardiac injury in severely burned rats.Methods Sixty Sprague-Dawley rats were divided into four groups（n=15）,including sham group（Group S,underwent sham burn and normal saline of 1 mL/h were given）,Burned group [Group C,third-degree burns over 30% total body surface area（TBSA） and normal saline of 1 mL/h for 6 h after burned were given],midazolam group [Group M,third-degree burns over 30% TBSA and midazolam of 0.6 mg/（kg·h） after burned were given],and dexmedetomidine group [Group D,third-degree burns over 30% TBSA and dexmedetomidine of 5 μg/（kg·h） after burned were given].Blood rheological parameters,and plasma tumor necrosis factor-alpha（TNF-α） and cTnI were assessed,while rat hearts was harvested for morphological examination 6 weeks after burned.Results Significant increase of ηb,Hct and cTnI were observed in Group C,M and D,when comparing with those in Group S（P0.05）.However,significant reduction of ηb,Hct and cTnI were revealed in Group D and M,when comparing with those in Group C（P0.05）.Furthermore,significantly more reduction in ηb and Hct were revealed in Group M than those in Group D（P0.05）.Significant elevation of plasma MDA and TNF-α with compromised SOD activity were revealed in Group C,M and D,when comparing with those in Group S（P0.01）.Meanwhile,significant reduction of TNF-α and cTnI with enhanced SOD activity were revealed in Group D when comparing with those in Group C（P0.01）.Furthermore,significant reduction of MDA and TNF-α were revealed in Group D,when comparing with those in Group M（P0.05）.Conclusion Dexmedetomidine effectively protects myocardium from burned-induced injury.
出处
《广东医学》
CAS
CSCD
北大核心
2012年第8期1050-1052,共3页
Guangdong Medical Journal
