摘要
目的:探讨华蟾素对体外培养的子宫内膜癌HHUA细胞侵袭性生长的影响和意义。方法:体外培养内膜癌HHUA细胞,以0.25、2.5和25 g/L的华蟾素干预后,四唑盐比色试验检测细胞增殖,流式细胞仪检测细胞凋亡,反转录-聚合酶链反应检测CD44s表达,transwell小室分析细胞的体外侵袭力。结果:华蟾素对内膜癌HHUA细胞的半数抑制浓度(IC50)为华蟾素生药(0.79±0.21)g/L。对照组CD44s mRNA表达为1.31±0.25、侵袭细胞数为60.50±2.52;0.25 g/L华蟾素对内膜癌HHUA细胞CD44smRNA表达和侵袭力无影响(均P>0.05);2.5 g/L华蟾素干预后,内膜癌HHUA细胞CD44s mRNA表达为1.02±0.23、侵袭细胞数为30.50±3.95,与对照组比较差异有统计学意义(均P<0.05);25 g/L华蟾素干预后,内膜癌HHUA细胞CD44s mRNA表达为0.97±0.39、侵袭细胞数为22.50±4.22,与对照组比较差异有统计学意义(均P<0.05);25 g/L和2.5 g/L华蟾素2个浓度组差异无统计学意义(均P>0.05)。结论:2.5 g/L华蟾素可抑制内膜癌HHUA细胞的侵袭性生长。
Objective：To investigate the effect of cinobufagin on the invasive growth of the endometrial carcinoma HHUA cells cultured in vitro. Methods：Endometrial carcinoma HHUA ceils were cultured in vitro ;after the action of einobufagin with different concentration, MTT method was used to detect the proliferation of HHUA cells,the cellular apop- tosis was tested by flow cytometry ,the expression of CD44s was measured by RT-PCR ,the ability of invasion Was compared with transwell chamber model. Results： Endometrial carcinoma HHUA ceils expressed CD44s mRNA of （1.31±0.25 ）,num- ber of invasive cell was 60.50±2.52 ;0.25 g/L cinobufagin didn＇t affect CD44s and invasive cell（P〉0.05） ; after the action of 2.5 g/L cinobufagin, the expression of CD44s was 1.02±0.23, number of invasive ceil was （30.50±3.95）, these deta were different from the control group of endometrial carcinoma HHUA cells significantly （P〈0.05） ;after the action of 25 g/L cinobufagin,the expression of CD44s was 0.97 ±0.39 ,number of invasive cell was 22.50 ±4.22, these data were different from the control group of endometrial carcinoma HHUA cells significantly （P〈0.05） ;the action of 25 g/L cinobufagin was similar to that of 0.25 g/L cinobufagin （P〉 0.05）. Conclusions：2.5 g/L cinobufagin inhibit the expression of CD44s mRNA and inhibit the invasive growth of the endometrial carcinoma HHUA cells.
出处
《国际妇产科学杂志》
CAS
2012年第2期199-201,共3页
Journal of International Obstetrics and Gynecology
