摘要
目的研究维生素D3(VD3)对电离辐射引起破骨细胞代谢改变的保护作用。方法 24只雄性C57BL/6小鼠随机分为对照组、γ射线辐照组和1000IU VD3注射处理的γ射线辐照组。酶联免疫吸附双抗体夹心法测定小鼠血清中小鼠肿瘤坏死因子α(TNF-α)、小鼠抗酒石酸酸性磷酸酶5b(TRAP-5b)、小鼠可溶性核因子-κB受体活化因子配体(sRANKL)、小鼠Ⅰ型胶原C端肽(CTX-1);采用甲基百里香酚蓝比色法测定血清中钙离子浓度。抗酒石酸酸性磷酸酶(TRAP)染色骨病理石蜡切片观察破骨细胞形态变化。用SPSS12.0统计软件分析数据。结果与对照组比,γ射线辐照组小鼠血清中TRAP-5b、CTX-1、sRANKL和TNF-α含量分别升高了37.30%、47.14%、19.55%和17.67%(P<0.05)。VD3注射处理的γ射线辐照组血清中TRAP-5b、CTX-1和sRANKL升高不明显(P>0.05)。VD3的保护率分别为42.06%、69.80%和68.12%,而TNF-α升高明显(P<0.05)。镜下γ射线辐照组小鼠骨内破骨细胞形态呈代谢增强改变;而VD3注射处理则明显改善γ射线辐照引起的破骨细胞的形态变化。结论 VD3通过降低破骨细胞活性关键因子sRANKL可以有效地减轻电离辐射引起的小鼠破骨细胞代谢增强。使用VD3是减轻放疗患者受电离辐射损害的一种潜在有效方法。
Objective To investigate the protective effect of vitamin D3（VD3） on mouse osteoclast activation induced by ionizing radiation.Method Twenty four male C57BL/6 mice were divided randomly into control group,γ-ray exposed group and injected 1000 IU VD3 combined with γ-ray exposed group.Double antibody sandwich enzyme-linked immunosorbent assay were performed to determine the levels of tartrate resistant acid phosphatase 5b（TRAP-5b）,type 1 collagen carboxy-terminal telopeptide（CTX-1）,soluble receptor activator of NF-kappa B ligand（sRANKL）,tumor necrosis factor α（TNF-α） in mice serum.Level of calcium（Ca） was determined by methylthymol blue colorimetric method.TRAP staining was performed to detect the histopathology of osteoclast.Results The levels of serum TRAP-5b,CTX-1,sRANKL and TNF-α in γ-ray exposed group were raised by 37.30%,47.14%,19.55% and 17.67% respectively as compared with control group（P0.05）.However,the levels of TRAP-5b,CTX-1 and sRANKL in VD3 combined with γ-ray exposed group were not significantly raised（P0.05）.The protective ratio of VD3 against γ-ray ionizing radiation was 42.06%,69.80% and 68.12% respectively,but TNF-α level was still higher（P0.05）.The increase of osteoclast activity in γ-ray exposed group was observed and the change was ameliorated after VD3 treatment.Conclusion VD3 played an efficient protective role in mitigating γ-ray ionizing radiation-induced osteoclast activation.Thus VD3 treatment may serve as a useful approach to protect the patients from the adverse effects during radiotherapy.
出处
《营养学报》
CAS
CSCD
北大核心
2012年第2期135-138,142,共5页
Acta Nutrimenta Sinica
