期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

脂肪酰胺水解酶在结直肠癌中的表达及临床意义

Expression of fatty acid amide hydrolase in colorectal cancer and its clinical significance
原文传递
导出
摘要 目的检测脂肪酰胺水解酶(fatty acid amide hydrolase,FAAH)在结直肠癌及正常肠黏膜中的表达,探讨其临床意义。方法应用Western blot和组织芯片技术结合免疫组化法检测结直肠癌组织以及对应正常肠黏膜中FAAH蛋白的表达。结果 FAAH在结直肠癌组织中的表达低于正常肠黏膜,169例结直肠癌组织中FAAH的阳性表达率显著低于正常肠黏膜(56.8%vs 97.4%,P<0.01),并与肿瘤的分化程度密切相关(P<0.01)。结论 FAAH在结直肠癌组织中低表达,在正常肠黏膜中高表达,在结直肠癌的恶性增殖中发挥着重要作用。 Objective To investigate the expression of fatty acid amide hydrolase（FAAH） in colorectal cancer and normal intestinal mucosa,and to explore the clinical significance of FAAH.Methods Western blotting and immunohistochemical staining were applied for the detection of FAAH expression in 169 cases of colorectal cancer tissues and 38 cases of normal intestinal mucosae.Results The expression level of FAAH in the colorectal cancer tissues was lower than that in the normal intestinal mucosae.The positive expression rate of FAAH in the colorectal cancer tissues was significantly lower than that in the normal intestinal mucosae（56.8% vs 97.4%,P0.01）.FAAH expression was significantly correlated with tumor differentiation degree（P0.01）.Conclusion FAAH,which is mildly expressed in colorectal cancer tissue but highly expressed in normal intestinal mucosa,plays an important role in the malignant proliferation of colorectal cancer.
作者 邓佳 谢赣丰 舒秋霞 王宁 梁后杰
机构地区 第三军医大学西南医院肿瘤科
出处 《第三军医大学学报》 CAS CSCD 北大核心 2012年第9期899-901,共3页 Journal of Third Military Medical University
关键词 结直肠癌 组织芯片 脂肪酰胺水解酶 colorectal cancer tissue microarray fatty acid amide hydrolase
分类号 R341.5 [医药卫生—基础医学] R730.23 [医药卫生—肿瘤]
  • 相关文献

参考文献14

  • 1Guzman M. Cannabinoids:potential anticancer agents[J].Nature Reviews Cancer,2003,(10):745-755.
  • 2Pushkarev V M,Kovzun O I,Tronko M D. Antineoplastic and apoptotic effects of cannabinoids.N-acylethanolamines:protectors or killers[J].Experimental Oncology,2008,(01):6-21.
  • 3Petrosino S,Di-Marzo V. FAAH and MAGL inhibitors:therapeutic opportunities from regulating endocannabinoid levels[J].Current Opinion in Investigational Drugs,2010,(01):51-62.
  • 4Izzo A A,Camilleri M. Cannabinoids in intestinal inflammation and cancer[J].Pharmacological Research,2009,(02):117-125.
  • 5Cencioni M T,Chiurchiu V,Catanzaro G. Anandamide suppresses proliferation and cytokine release from primary human T-lymphocytes mainly via CB2 receptors[J].PLoS One,2010,(01):e8688.doi:10.1371/journal.pone.0008688.
  • 6Hiley C R,Hoi P M. Oleamide:a fatty acid amide signaling molecule in the cardiovascular system[J].Cardiovascular Drug Reviews,2007,(01):46-60.
  • 7Tree K,Caravagna C,Hilaire G. Anandamide centrally depresses the respiratory rhythm generator of neonatal mice[J].Neuroscience,2010,(04):1098-1109.
  • 8De-Petrocellis L,Bisogno T,Ligresti A. Effect on cancer cell proliferation of palmitoylethanolamide,a fatty acid amide interacting with both the cannabinoid and vanilloid signalling systems[J].Fundamental and Clinical Pharmacology,2002,(04):297-302.
  • 9Nithipatikom K,Isbell M A,Endsley M P. Anti-proliferative effect of a putative endocannabinoid,2-arachidonylglyceryl ether in prostate carcinoma cells[J].Prostaglandins & Other Lipid Mediators,2011,(1/2):34-43.
  • 10Mimeault M,Pommery N,Wattez N. Anti-proliferative and apoptotic effects of anandamide in human prostatic cancer cell lines:implication of epidermal growth factor receptor down-regulation and ceramide production[J].Prostate,2003,(01):1-12.

二级参考文献34

  • 1Wilson RI, Nicoll RA. Endocannabinoid signaling in the brain. Science 2002; 296:678-682.
  • 2Roth MD, Baldwin GC, Tashkin DP, Effects of delta-9-tetrahydrocannabinol on human immune function and host defense. Chem Phys Lipids 2002; 121:229-239.
  • 3Pinto L, Izzo AA, Cascio MG, Bisogno T, Hospodar- Scott K, Brown DR, Mascolo N, Di Marzo V, Capasso F. Endocannabinoids as physiological regulators of colonic propulsion in mice. Gastroenterology 2002; 123:227-234.
  • 4Mascolo N, Izzo AA, Ligresti A, Costagliola A, Pinto L, Cascio MG, Maffia P, Cecio A, Capasso F, Di Marzo V. The endocannabinoid system and the molecular basis of paralytic ileus in mice. FASEB J 2002; 16:1973-1975.
  • 5Wright K, Rooney N, Feeney M, Tate J, Robertson D, Welham M, Ward S. Differential expression of cannabinoid receptors in the human colon: cannabinoids promote epithelial wound healing. Gastroenterology 2005; 129:437-453.
  • 6Storr M, Sibaev A, Marsicano G, Lutz B, Schusdziarra V, Timmermans JP, Allescher HD. Cannabinoid receptor type 1 modulates excitatory and inhibitory neurotransmission in mouse colon. Am J Physiol Gastrointest Liver Physiol 2004; 286: G110-G117.
  • 7Maccarrone M, Gasperi V, Fezza F, Finazzi-Agro A, Rossi A. Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone. Eur J Biochem 2004; 271:4666-4676.
  • 8Izzo AA, Capasso F, Costagliola A, Bisogno T, Marsicano G, Ligresti A, Matias I, Capasso R, Pinto L, Borrelli F, Cecio A, Lutz B, Mascolo N, Di Marzo V. An endogenous cannabinoid tone attenuates cholera toxin-induced fluid accumulation in mice.Gastroenterology 2003; 125:765-774.
  • 9Mang CF, Erbelding D, Kilbinger H. Differential effects of anandamide on acetylcholine release in the guinea-pig ileum mediated via vanilloid and non-CB1 cannabinoid receptors. Br J Pharmacol 2001; 134:161-167.
  • 10Steffens M, Szabo B, Klar M, Rominger A, Zentner J, Feuerstein TJ. Modulation of electrically evoked acetylcholine release through cannabinoid CB1 receptors: evidence for an endocannabinoid tone in the human neocortex. Neuroscience 2003; 120: 455-465.
第三军医大学学报
2012年 第9期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部