摘要
为了建立一种高效液相色谱法测定奥氮平及其口崩片的含量,采用Venusil ASB C18色谱柱(4.6 mm×150 mm,5μm)进行分析,流动相为缓冲液(pH=2.5)∶乙腈=45∶55(体积比);检测波长为259 nm;柱温为25℃;流速为1.5 mL.min-1;进样量为20μL.结果表明:奥氮平在20.33～203.30μg.mL-1范围内线性关系良好(r=0.999 8),平均回收率为100.12%(RSD=0.33%),检测限为1 ng;该方法灵敏快速、重复性好、专属性强,可用于非经典抗精神病药奥氮平及其口崩片的含量测定.
To establish a high performance liquid chromatography method for determining the content of olanzapine and in orally disintegrating tablets,the best HPLC conditions were set as follows: a Venusil ASB C18(4.6 mm×150 mm,5 μm)column,mobile phase of buffer solution(pH=2.5)-acetonitrile(45∶ 55),ultraviolet absorption detector of 259 nm,column temperature of 25 ℃,flow rate of 1.5 mL·min-1,injection volume of 20 μL.The results show that the linearity of olanzapine is in the range of 20.33 to 203.30 μg·mL-1(r=0.9998),the average recovery is 100.12% with relative standard deviation(RSD) of 0.33%,the limit of detection(LOD) is 1 ng.The method is sensitive,quick,repeatable,and thus it can be provided for determining the content of olanzapine and in orally disintegrating tablets.
出处
《武汉工程大学学报》
CAS
2012年第4期11-14,68,共5页
Journal of Wuhan Institute of Technology
基金
"十二五"国家科技重大专项课题资助(2012ZX09301003-001)
关键词
高效液相色谱法
奥氮平
口崩片
含量测定
high performance liquid chromatography
olanzapine
orally disintegrating tablets
quantitative determination
