摘要
以三蕊柳的休眠芽为材料,进行了萌动芽的生长,不定芽的分化,试管苗的继代培养与留茬培养,生根苗扦插和移植的研究,成功地建立起三蕊柳快速繁殖技术.研究结果证明:MS+BA0.1 mg.L-1+GA 1.0 mg.L-1+IAA 0.2 mg.L-1是休眠芽生长培养的理想培养基;1/2MS+BA 0.6 mg.L-1+IAA 0.2 mg.L-1+NAA 0.1mg.L-1是生长芽分化培养的理想培养基;1/2MS+BA 0.4mg.L-1+AgNO30.8 mg.L-1+GA30.5 mg.L-1+IAA 0.3 mg.L-1+NAA 0.1 mg.L-1是不定芽分化继代培养和留茬继代培养的理想培养基;移植到河滩上的试管苗生长旺盛,呈丛生状.
In this paper,the growing and differentiation of adventitious bud,subculture of test-tube plant,cattaging and transplanting of rooted seedling are studied by using dormant bud of salix triandra var.triandra.The results showed that MS＋BA 0.1 mg·L-1＋GA 1.0 mg·L-1＋IAA 0.2 mg·L-1 is suitable for the growth of dormant bud;1/2MS＋BA 0.6mg·L-1＋ IAA 0.2 mg·L-1＋NAA 0.1 mg·L-1 is suitable for differentiation of the bud;1/2MS＋BA 0.4 mg·L-1＋AgNO3 0.8 mg·L-1＋GA3 0.5 mg·L-1＋ IAA 0.3 mg·L-1＋NAA 0.1mg·L-1 is suitable for subculture of the adrentitious bud.The seedling grew well and thickly on the flood land by transplanting.
出处
《哈尔滨师范大学自然科学学报》
CAS
2011年第3期76-79,共4页
Natural Science Journal of Harbin Normal University
基金
辽宁省教育厅科学技术研究项目资助(2008353)
关键词
三蕊柳
组织培养
快速繁殖
Salix triandra var.triandra
Tissue Culture
Rapid propagation
