摘要
目的建立HPLC法测定安乃近片的含量。方法采用KromasilC18柱(250.0mm×4.6mm,5μm),以磷酸盐缓冲液(pH为7.0)-甲醇(72:28)为流动相,UV检测波长254nm,流速1.0mL/min。结果安乃近在104.4～417.6μg/mL范围内呈良好的线性关系(r=0.9999),平均回收率为99.7%,RSD=0.64%。结论本法测定安乃近片的含量科学、准确、简便,可用于安乃近片的质量控制。
Objective To establish an HPLC method for the determination of Metamizole SodiumTablets.Methods Metamizole Sodium was separated on Kromasil C18（250.0 mm×4.6 mm,5 μm） and detected at 254 nm,with phosphate buffer（pH was 7.0）-methanol（72：28） as mobile phase,the flow rate was 1.0 mL/min.Results Metamizole Sodium presented a good linear correlation within the range of（104.4-417.6） μg/mL（r = 0.999 9）.The average recovery was 99.7%,with RSD=0.64%.Conclusion This method is scientific,accurate and simple.This study provides the reference to establish quality standard for Metamizole Sodium Tablets.
出处
《中国当代医药》
2012年第11期53-54,共2页
China Modern Medicine
关键词
安乃近片
含量
HPLC
测定
Metamizole Sodium Tablets
Content
HPLC
Determination
