摘要
为了研究饲粮蛋白水平对猪骨骼肌中CaN,NFAT和CaM蛋白表达量及肌肉剪切力的影响,探讨各个蛋白表达与剪切力之间的关系。试验选用90头约50kg的杜洛克×长白×大白三元杂交猪(公母各半,公猪去势),随机分为3个处理组,每个处理组3个重复,每个重复10头,分别饲喂蛋白质水平为12%、16%和20%的饲粮,各饲粮能量及氨基酸模式保持一致,饲养58d后屠宰取样,用蛋白质免疫印迹法测定猪背最长肌中CaN、NFAT和CaM的蛋白相对表达量。结果表明:饲粮中不同蛋白质水平对猪骨骼肌中CaN蛋白相对表达量影响极显著(p<0.01),对NFAT和CaM蛋白相对表达量无显著影响(p>0.05),但随着饲粮中蛋白水平升高而升高;CaN、NFAT和CaM在骨骼肌中蛋白相对表达量的变化呈正相关,且相关性非常明显(p<0.01);CaN、NFAT和CaM的蛋白相对表达量越高,骨骼肌剪切力值越高,但相关性不明显(p>0.05)。提高饲粮中的蛋白质水平将促进CaN、NFAT和CaM的表达,从而促进钙调磷酸酶-NFAT信号通路发挥作用,钙调磷酸酶-NFAT信号通路可能影响肌肉嫩度。
This study was conducted to investigate the effects of dietary protein levels on CaN, NFAT and CaM expression of pig skeletal muscle and muscle shear force and the relationship between the protein expressions and the shear force in pig skeletal muscle. Ninety crossed-bred （DurocxLandracexYorkshine） pigs with an average weight of 50 kg were randomly assigned to three dietary treatments groups with three replicates and ten pigs with each pen, and fed with diets of three protein levels （12%, 16% and 20%） respectively. The energy level and amino acid pattern in the three diets were kept the same. The whole feeding trial lasted for 58 days. Then Western Blotting was used to determine the proteomics relative expression of CaN, NFAT and CaM in skeletal muscle.The results showed different levels of dietary protein had significant effects on the CaN relative quantity in skeletal muscle （p〈0.O1）, while less significant effects on the NFAT and CaM relative quantity （p〉0.05）, and increased as diet protein levels increased. There were positive correlations among CaN, NFAT and CaM, and the correlation was significant （p〈 0.01）. When CaN, NFAT and CaM relative quantity increased, the shear force in the skeletal muscle increased too, but the correlation was not significant（p〉0.05）. The results suggested that increasing of the diet protein level will enhance the expression of CaN, NFAT and CaM, and strengthen the regulation of Calcineurin/NFAT signal pathway. Calcineurin/NFAT signal pathway may affect the shear force in muscle.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2011年第6期703-708,共6页
Journal of Shenyang Agricultural University
基金
国家自然科学基金项目(30972112)
关键词
肥育猪
背最长肌
CAN
NFAT
CAM
剪切力
finishing pig
longissimus dorsi
calcineurin
NFAT
calmodulin
shear force
