摘要
利用2011年夏初(5月30日至6月3日)的低温天气,对粳稻Nipponbare、籼稻Kasalath及其杂交后回交衍生的98个BC1F5回交重组自交系群体进行苗期自然低温处理。通过分析其对应的包含245个分子标记的连锁图谱与苗期抗冷能力的关系,共检测到控制苗期抗冷的2个主效QTL和1个微效QTL,主效QTL分别是位于第1染色体上的qCTS1和位于第3染色体上的qCTS3;微效QTL是位于第8染色体上的qCTS8。其中,两个主效QTL的抗冷等位基因来自于粳型亲本日本晴,而微效QTL的抗冷等位基因来自于籼型亲本Kasalath。三者的表型贡献率分别为30.8%、22.7%和10.3%。这些QTL将为水稻抗冷设计育种提供"元件",同时为阐明水稻抗冷分子机制提供基础信息。
Nipponbare （japonica）, Kasalath （indica） and ninety-eight backcross recombinant inbred lines （BC1F5） derived from the cross between them and its linkage map including 245 molecular markers were used to detect QTL controlling cold tolerance at seedling stage by using the low temperature weather in early summer （from 30 May to 3 June） of 2011. Two major QTLs and one minor QTL were identified. The major QTLs qCTS1 and qCTS3 were detected on chromosome 1 and chromosome 3, respectively. The minor QTL qCTS8 was identified on chromosome 8. Cold tolerance alleles of the two major QTLs were from japonica parent Nipponbare and that of minor QTLs was from indica parent Kasalath. The phenotypic variations （VE） explained of each individual QTL were 30.8%, 22.7% and 10.3%. These QTLs provide ＂elements＂ and basic-information for cold tolerance design breeding and elucidation of cold tolerance molecular mechanism in rice.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2011年第6期654-657,共4页
Journal of Shenyang Agricultural University
基金
国家自然科学基金项目(31100881)
中国博士后科学基金项目(2011M501077)
关键词
水稻
苗期
抗冷
数量性状基因位点
rice
seedling stage
cold tolerance
quantitative trait loci
