摘要
为了进一步明确放线菌11-3-1菌株的生防效果和分类地位,采用平板对峙法和活体组织法测定其抑菌效果。放线菌11-3-1菌株对供试的15种植物病原真菌菌丝生长均有不同程度的抑制作用,尤其对油菜菌核病菌具有明显抑制作用,抑菌带可达到19.67 mm。显微镜观察显示,11-3-1菌株对油菜菌核病菌菌丝生长有明显影响,可致使油菜菌核病菌菌丝体畸形、扭曲和原生质浓缩外渗等。11-3-1菌株发酵原液对油菜菌核病的室内药效达96.91%,效果显著优于对照组和药剂对照组。根据形态观察、生理生化特性和16S rDNA序列比对分析,初步鉴定该菌株为黄色长孢链霉菌Streptomyces longisporoflavus。
In order to ensure the efficacy and its taxonomic status of actinomycete strain 11-3-1,agar block method and tissue culture method were used to test the antifungal activity.The results showed that the strain displayed a broad-spectrum inhibitory activity against 15 plant pathogenic fungi,especially to Sclerotinia sclerotiorum,the width of inhibition zones was up to 19.67mm.Strain 11-3-1 induced alterations on hyphae morphology of S.sclerotiorum,such as distortion,cytoplasm exosmosis and deformation.Tissue culture method tests showed that the inhibitions rates of the fermentation broth was 96.91% to S.sclerotiorum,and fermentation broth had a better control effect than CK group and fungicide group.According to the morphological,physiological and biochemical characteristics,and 16S rDNA sequence,the strain 11-3-1 was identified as Streptomyces longisporoflavus.
出处
《植物保护学报》
CAS
CSCD
北大核心
2012年第2期97-102,共6页
Journal of Plant Protection
基金
国家公益性行业(农业)科研专项(200903052)
关键词
放线菌
抑菌活性
分类鉴定
油菜菌核病菌
actinomyces
inhibitory activity
identification
Sclerotinia sclerotiorum
