摘要
目的研究山东省历城区三带喙库蚊成蚊及幼虫对常用杀虫剂的敏感性,了解其对常用杀虫剂的抗药性水平,为三带喙库蚊的化学控制提供理论依据。方法在三带喙库蚊的发生高峰期,大量采集野外三带喙库蚊成蚊,用小白鼠供血获得子一代幼虫和现场捕获且带回实验室产一次卵后的雌成蚊为试虫;用WHO推荐的药膜接触法、幼虫浸渍法,分别测定三带喙库蚊成蚊及幼虫对常用杀虫剂的敏感性;获得成蚊的击倒率和死亡率,幼虫对常用杀虫剂的LC50及其95%置信限(95%CI),比较其抗药性程度。结果三带喙库蚊成蚊对0.05%溴氰菊酯(1 h)、0.05%高效氯氰菊酯(1 h)、0.25%氯菊酯(3 h)、0.45%氯菊酯(3 h)、0.05%氯氰菊酯(1 h)、0.45%胺菊酯(1 h)等拟除虫菊酯类杀虫剂的死亡率分别为85.0%、74.6%、38.0%、92.9%、36.2%和43.6%;对1%杀螟硫磷(2 h)死亡率为16.1%;对0.1%残杀威(2 h)死亡率为16.9%;对4%DDT(4 h)死亡率为36.4%。三带喙库蚊幼虫对溴氰菊酯、高效氯氰菊酯、氯菊酯的LC50及其95%CI分别为0.0046(0.0037～0.0055)、0.0113(0.0092～0.0136)和0.0325(0.0289～0.0369)mg/L;DDVP、双硫磷、杀螟硫磷、马拉硫磷的LC50及其95%CI分别为4.9047(3.8612～6.4215)、9.5733(7.4644～12.5296)、1.2694(1.0280～1.5055)和0.7750(0.6060～0.9842)mg/L;仲丁威的LC50及其95%CI为4.4237(3.7406～5.2769)mg/L。与已发表文献的敏感品系比较,三带喙库蚊幼虫对溴氰菊酯、高效氯氰菊酯和氯菊酯相对抗性倍数分别为11.5、1.9和8.8倍,对DDVP、双硫磷、杀螟硫磷和马拉硫磷的相对抗性倍数分别为76.6、7977.8、256.4和84.2倍。结论山东省历城区三带喙库蚊成蚊对常用杀虫剂均已产生抗性,以有机磷类和氨基甲酸酯类最为严重;幼虫对有机磷类杀虫剂的抗性水平较高,双硫磷最高。
Objective To investigate the susceptibility and resistance of larvae and adult of Culex tritaeniorhynchus in Licheng district,Shandong province to the commonly used insecticides,providing the basis for chemical control of the insects.Methods A large number of adult Cx.tritaeniorhynchus were collected outdoors in the peak seasons.The first filial generation of larvae was obtained by feeding the collected adults with blood from mice.The larvae obtained and the adult female mosquitoes,which had been captured in the field and had laid the first eggs in the lab,were used as test insects.The susceptibility of the adult and larvae to commonly used insecticides were determined with the drug membrane contact method and the impregnation method respectively,as the WHO protocol,with the knockdown rate and mortality of the adult mosquitoes and the LC50（95%CI） for the larvae calculated and their resistance levels to commonly used insecticides compared.Results The mortality of the adult exposed to 0.05% deltamethrin（contact for 1 h）,0.05% beta-cypermethrin（1 h）,0.25% permethrin（3 h）,0.45% permethrin（3 h）,0.05% cypermethrin（1 h）,0.45% tetramethrin（1 h）,1% fenitrothion（2 h）,0.1% propoxur（2 h） and 4%DDT（4 h） beyond 24 h were 85.0%,74.6%,38.0%,92.9%,36.2%,43.6%,16.1%,16.9% and 36.4%,respectively.The LC50 and the corresponding 95%CI of deltamethrin,beta-cypermethrin,and permethrin for Cx.tritaeniorhynchus larvae were 0.0046（0.0037-0.0055）,0.0113（0.0092-0.0136）,0.0325（0.0289-0.0369） mg/L,respectively;the LC50（95%CI） of dichlorvos,temephos,fenitrothion and malathion and BPMC were 4.9047（3.8612-6.4215）,9.5733（7.4644-12.5296）,1.2694（1.0280-1.5055）,0.7750（0.6060-0.9842） and 4.4237（3.7406-5.2769） mg/L,respectively.Compared to the susceptible strains reported in previous literature,the resistance coefficients of the larvae of Cx.tritaeniorhynchus were 11.5,1.9 and 8.8 to deltamethrin,beta-cypermethrin and permethrin,respectively,and 76.6,7977.8,256.4 and 84.2 to dichlorvos,temephos,fenitrothion and malathion,respectively.Conclusion Adult Cx.tritaeniorhynchus in Licheng district has developed resistance to the commonly used insecticides,with a very high resistance seen to organophosphate and carbamate pesticides in the adult.The resistance of larvae to temephos is highest among the organophosphates.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2012年第2期89-92,共4页
Chinese Journal of Vector Biology and Control
基金
国家科技重大专项课题(2008ZX10004-010)~~
关键词
三带喙库蚊
常用卫生杀虫剂
抗药性
Culex tritaeniorhynchus
Commonly used insecticides for public health
Insecticide resistance
