摘要
以‘香山水蜜桃’为试材,研究叶面喷施DA-6(二烷氨基乙醇羧酸酯N,N-diethylaminoethyl hexanote)对叶绿素(Chlorophyll,Chl.)合成过程中前体物质的含量以及合成关键酶活性的影响,探讨DA-6提高桃叶片Chl.含量的可能原因。结果表明:生长季叶面喷施20mg·L-1DA-6显著提高了Chl.的含量,Chl.b含量的提高尤为显著,降低了Chl.的前体物质5–氨基酮戊酸(5-aminolevulinic acid,ALA)含量,增加了后续中间产物胆色素原(porphobilinogen,PBG)、尿卟啉原Ⅲ(uroporphyrinogeⅢ,UrogenⅢ)含量,表明DA-6处理促进了ALA向PBG的转化和PBG向UrogenⅢ的转化。以上两步骤的关键酶5–氨基酮戊酸脱水酶(5-aminolevulinate dehydratase,ALAD)和尿卟啉原Ⅲ合酶(uroporporphyrinogn Ⅲ synthase,UROS)的活性在处理后显著提高。叶面喷施DA-6是通过提高ALA向PBG和PBG向UrogenⅢ的转化效率,促进Chl.的合成,使Chl.含量增加。
Taken‘Xiangshan Honey’peach as the material,the change of chlorophyll precursor contents and activity of the key enzymes in chlorophyll biosynthesis pathway after DA-6 （N-diethylaminoethyl hexanote）application were investigated the underlying mechanism of DA-6 induced leaf chlorophyll content increase. Compared with the control,20 mg · L-1 foliar DA-6 spray significantly increased the contents of chlorophylls,especially that of chlorophyll b,and chlorophyll intermediate products porphobilinogen（PBG）and uroporphyrinoge（Urogen Ⅲ）,whereas decreased the amount of chlorophyll precursor 5-aminolevulinic acid（ALA）. The results suggested the transformation from ALA to PBG and PBG to Urogen Ⅲ was up-regulated. The key enzymes catalyzing the two reactions are 5-aminolevulinate dehydratase（ALAD）and uroporporphyrinogn Ⅲ synthase（UROS）, their enhanced activity after DA-6 foliar spraying was assayed. Our study demonstrated that foliar spray DA-6 increased the conversion efficiency of ALA to PBG and PBG to Urogen Ⅲ,up-regulated the chlorophyll biosynthesis pathway and resulted in higher chlorophyll content in peach leaves.
出处
《园艺学报》
CAS
CSCD
北大核心
2012年第4期621-628,共8页
Acta Horticulturae Sinica
基金
农业部公益性行业项目(nyhyzx07-025)
关键词
桃
DA-6
叶绿素
合成
前体物质
关键酶
酶活性
peach
DA-6
chlorophyll
biosynthesis
precursors
key enzyme
enzyme activity
