摘要
目的:建立同时测定忍冬与红腺忍冬中绿原酸、芦丁和槲皮素的HPLC分析方法。方法:依利特Hypersil ODS(4.6 mm×250 mm,5μm),流动相乙腈-0.05%磷酸梯度洗脱,检测波长327 nm,柱温25℃,进样10μL,流速1.0 mL·min-1。结果:线性范围内3种成分的标准曲线呈良好的线性关系,加样回收率在97%～101%,精密度和重复性的RSD均符合定量分析要求。结论:该方法准确、简便,具有良好的重复性和稳定性,可用于忍冬及红腺忍冬药材的质量控制。
Objective：HPLC method was establisheet for simultaneous determinaton of chlorogenic acid,rutin and quercetin in honeysuckle and red gland honeysuckle.Method： The separation was performed on a Elite Hypersil ODS C18 column（4.6 mm×250 mm,5 μm）.acetonitril-0.05% H3PO4 was used as the mobile phase.The detection wavelength was at 327 nm.The temperature was maintained at 25 ℃.The volume was 10 μL.The flow rate was 1.0 mL·min-1.Result： Within Linear range,the three components of the standard curve are in good linear relationship within linear range.The average recovery was 97%-101% recovery.RSD of precision and repeatability accord with quantitative analysis requirements.Conclusion： This method is accurate,simple,with good repeatability and stability and can be used for honeysuckle and red gland honeysuckle quality control.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第8期83-85,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西科技厅科技攻关项目(桂科攻0815005-2-5)
广西山银花生长环境
生长特征与药材质量相关性研究(GZZC0907)
关键词
忍冬
红腺忍冬
绿原酸
芦丁
槲皮素
honeysuckle
red gland honeysuckle
chlorogenic acid
rutin
quercetin
