摘要
对22份枸杞属种质材料进行RAPD分析。研究结果表明,32个引物在22份种质中共扩增出了204条带,其中162条为多态性条带,多态性条带的比例为79.41%。UPGMA聚类分析结果表明,RAPD分子标记的聚类结果与枸杞属形态分类基本相似,能将种间亲缘关系划分出,但是对变种与种间关系的区分不佳。
The genomic DNA of 22 wolfberry germplasms were tested with RAPD using 32 arbitrary lO-mer primers which were screened from 100 primers.The 162 polymorphic bands of 204 RAPD bands were obtained with thepercentage of polymorphic bands 79.41%. UPGMA cluster analysis based on RAPD markers data.The result showed that the RAPD approach was efficient in generating accurate information on genetic background, relationship and evolution of wo｜iberry gcrmplasms.
出处
《广东农业科学》
CAS
CSCD
北大核心
2012年第5期112-113,127,共3页
Guangdong Agricultural Sciences
基金
国家自然科学基金(31060206
31060104)
国家科技支撑计划项目(2009BAI72B01)
关键词
枸杞
种质
RAPD
wolfberry
germplasms
RAPD
