摘要
目的 比较免疫印迹 (IB)和盐裂皮肤间接免疫荧光 (IIF)检测自身免疫性表皮下大疱病 (SABD)基底膜带自身抗体 (BMZ Ab)的敏感性和特异性。方法 分别应用IIF和IB技术对 97例SABD患者血清中IgG型或IgA型BMZ Ab进行检测。结果 IIF法阳性率 75 .3 % ,免疫印迹法阳性率 79.4% ,两者在检测灵敏度上无显著性差异。结论 二者均可用于SABD中BMZ Ab的检测 ,二者联用对于提高BMZ
Objective To compare the sensitivity and specificity of immunoblotting (IB) and saltsplit skin indirect immunofluorescence (IIF) in detecting basement membrane zone antibodies (BMZAb) in subepidermal autoimmune blistering diseases (SABD).Method IB and saltsplin skin IIF were performed to detect IgG or IgA BMZAbS of 97 cases with SABD.Results The positive rate of IIF was 75.3% while that of IB was 79.4%. There was no difference between the two methods in detecting BMZAbs. Conclusion Both two methods could be used in detecting BMZAbs in SABD sera. The combination of two methods could promote the diagnoisis of SABD.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2000年第3期145-146,共2页
The Chinese Journal of Dermatovenereology
基金
国家自然科学基金资助课题!(编号39570659)
安徽省自然科学基金资助课题!(编号97424005)
关键词
自身免疫性
表皮下大疱病
免疫印迹
自身抗体
Subepidermal autoimmune blistering diseases Immunoblotting Salt-split skin indirect immunofluorescence Basement membrane zone autoantibody
