摘要
聚β-羟基丁酸酯(PHB)作为一种新型的生物可降解高分子材料,日益受到人们的广泛重视。利用12C6+离子束对一株聚β-羟基丁酸酯(PHB)产生菌———芽孢杆菌Z-3进行诱变,然后进行筛选,从大量突变株中最终选育出一株PHB高产菌株A11,其PHB产量达到1.404 g/L,是出发菌株的1.33倍。研究结果揭示,重离子诱变育种技术具有良好的诱变效果,是一种有效的微生物诱变育种方法和途径。
As a new biodegradable polymer materials,Poly-β-hydroxybutyrate(PHB) has attracted wide attention from people day by day.This study adopts heavy-ion mutation breeding technology,using 12C6+ ion beams to mutate the PHB producing strain-Bacillus Z-3,then screen a PHB high yielding strain A11 from a mount of mutant strains.Its PHB output can reach 1.404 g/L.This is 1.33 times as compared with the original strain.The results indicate that heavy-ion mutation breeding technology has good mutagenic effects.It is an effective method and way to microbial mutation breeding.
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2012年第2期95-100,共6页
Journal of Agricultural Science and Technology
基金
甘肃省科技支撑计划项目(090NKCA079)
国家自然科学基金项目(30870384)
铁道部科技研究开发计划课题(2010Z001-E)资助
关键词
聚Β-羟基丁酸
重离子
诱变
选育
高产菌株
poly-β-hydroxybutyrate
heavy ion
mutagenesis
selective breeding
high-yield strain