摘要
目的:比较伊马替尼敏感及耐药K562(K562/Ima)细胞P-糖蛋白、缺氧诱导因子1α和鞘氨醇激酶表达的差异。方法:取对数生长期的K562和K562/Ima细胞,采用不同质量浓度的伊马替尼分别处理48h,应用MTT法计算耐药倍数;另取上述2种细胞,分别采用Western blot、RT-PCR及同位素掺入等方法检测2种细胞中P-糖蛋白、缺氧诱导因子1α和鞘氨醇激酶的表达。结果:相对于K562细胞,K562/Ima细胞的耐药倍数为24。在K562/Ima细胞中,P-糖蛋白、缺氧诱导因子1α和鞘氨醇激酶的表达均高于K562细胞。结论:K562/Ima的耐药机制与P-糖蛋白、缺氧诱导因子、鞘氨醇激酶等基因的表达上调有关。
Aim：To compare the expressions of P-glycoprotein（P-gp）,HIF-1α,and SPK between chronic myeloid leukemia cell line K562 and imatinib-resistance K562（K562/Ima）cell line.Methods：K562 and K562/Ima cells were treated with different doses of imatinib for 48 h,and MTT was used to calculate the resistance fold.Western blot,RT-PCR and TLC were used to detect the expressions of P-gp,HIF-1α and SPK in K562 and K562/Ima cells,respectively.Results：The results revealed K562/Ima cells showed 24 fold resistance to imatinib compared with K562 cells.The expressions of P-gp,SPK and HIF-1α were up-regulated in K562/Ima cells.Conclusion：It is concluded that the resistance of K562/Ima cells is related to the high expressions of P-gp,SPK and HIF-1α.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2012年第2期153-155,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
国家自然科学基金资助项目30472082
