摘要
用自行建立的一株小鼠T淋巴细胞性白血病克隆细胞株(LAC—1),经腹腔免疫同系SW—1小鼠,诱导出表型为Thy1^+、Lyt2^+的T杀伤细胞(Tc).在含TCGF的完全培养液中,建立了体外长期存活和扩增的Tc细胞系,它仅对LAc—1及其母系L783白血病细胞呈现特异性杀伤活性,这一杀伤作用受到MHC的限制.
Cytotoxic T lymphocytes (CTL) derived from the spleen of SW-1 mice immunized in vivo with syngeneic mouse leukemia clonal cells LAC-1 were cultured in TCGF containing media and tested for cytotoxicity against LAC-1 cells in a routine ^(51)Cr-release assay. The experimental results showed that the CTL cell line was proved to be Thy 1 and Lyt 2, and therefore they were considered to be of T cell lineage. When CTL were expanded in the presence of TCGF, an augmented cytolytic activity in parallel with the increase of Thy 1 and Lyt 2 cells was shown. This cytotoxic T cell line had been maintained in a long-term culture for nearly 1 year and retained the initial reactivity. Specificity studies of the CTL showed that the cell line was highly specific for LAC-1 cells and its parent line L783 Leukemic cells and the cytolytic effect was H-2 restriction. The cytotoxicity of the CTL could be blocked by conventional or monoclonal antibodies against cell surface antigen (TSA) of LAC-1 cells. Antisera against H-2 antigens, shared by LAC-1 cells, also showed blocking activity.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1990年第3期157-160,共4页
Chinese Journal of Immunology
基金
国家教委博士点基金
关键词
肿瘤
克隆细胞株
T杀伤细胞
Mouse T lymphocytic leukemic cell clone
Tumor specific cytotoxic T cells
T cell growth factor(TCGF)
MHC restriction
