摘要
目的:通过观察"百会透曲鬓"针刺法对脑出血急性期大鼠模型脑组织中白介素-6(IL-6)蛋白表达的影响,研究本针刺法对脑出血后继发性脑损伤的神经保护机制,从而指导临床实践。方法:选用60只雄性Wistar大鼠,随机分为空白组、模型组、针刺组、西药组(脑复康)。分别于脑出血急性期大鼠模型造模成功后6h,2天,7天三个时间点并完成相应治疗,治疗后进行神经功能学评分,然后处死大鼠,取大鼠脑组织,用免疫组化分析法(IHC)检测脑组织中IL-6蛋白表达的平均灰度值,在光镜下观察脑组织形态学变化。结果:针刺治疗能减少神经功能缺损评分,造模后2天及7天后,针刺组与模型组比较有显著性差异(P<0.01);针刺治疗能降低IL-6阳性细胞平均灰度值,造模后2天及7天后,针刺组与模型组比较有显著性差异(P<0.01);光镜下脑组织病理改变结果显示,针刺组与模型组有差异。结论:"百会透曲鬓"针刺法可明显改善大鼠的神经功能学评分,有利于脑出血后肢体运动功能的恢复。在脑出血急性期,针刺"百会透曲鬓"穴可以通过下调IL-6的蛋白表达,减轻脑出血后炎性反应导致的继发性脑损伤程度,从而改善脑组织缺血缺氧状态及保护受损伤的神经元细胞。
Objective ： By observing the BaiHui - QuBin scalp - acupuncture on acute cerebral hemorrhage in the rat model of brain tissue IL -6 protein expression, to study the acupuncture on the cerebral hemorrhage secondary brain injury after the neuro protective mechanisms in order to guide clinical practice. Method ： 1. To- tally 60 male wistar rats were randomly allocated into four group：blank group （n = 6 ） , intracerebral hemorrhage control group （ n = 18 ）, acupuncture group （ n = 18 ）, and western medicine （pisaeetam） group （ n = 18 ）. 2. The rat models of acute intracerebral hemorrhage were made. 3. The acupuncture point ： BaiHui point to Qu- Bin point, which could penetrate three regions of the cupula, forehead and temple. 4. The Berderson neural deficit scores were tested at three time points （6h,2d,7d after hemorrhage model establishment）before the rat models were killed, then the brains were removed and indexes were detected. 5. The average gray scale of In-terleukin- 6 protein expression by immunohistoc -hemical method were checked, and the morphologic change rologic on light microscope was observed. 6. Statistical treatment was made. Results ： 1. The results of the neu- deficit score of acupuncture group was better than intracerebral hemorrhage group and western medicine group for the first two days. Acupuncture group and western medicine group were better than intracerebral hemorrhage group after seven days. 2. The results of masculine cell in the average gray scale of Interleukin - 6 in untreated control group were different from intracerebra -1 hemorrhage group （ICH）. In two days, the av- erage gray scale of Interleukin - 6 in aeupu - ncture group was lower than that in intracerebral hemorrhage group. There was also difference between acupuncture group and western medicine group. Acupuncture group and western medicine group were lower than those in intracerebral hemorrhage group, and there was a difference between acupuncture group and western medicine group in 7 days. 3. The resuh,; of the brain pathological change in acupuncture group group. Conclusion ： 1. BaiHui and western medicine group were different from those in intracerebral hemorrhage -QuBin acupuncture can obviously raise the B -erderson neural deficit scores, and have the benefit for improvement of extremity function after intracerebral hemorrhage. 2. Study shows that acute phase of cerebral hemorrhage, BaiHui - QuBin scalp - acupuncture therapy can reduce IL - 6 protein expression, reduce the inflammatory response after cerebral hemorrhage caused by the extent of secondary brain injury, ischemic brain tissue in order to improve status and the protection of hypoxic injury in neurons.
出处
《中医药信息》
2012年第2期86-90,共5页
Information on Traditional Chinese Medicine
基金
国家自然科学基金项目(30772840)
哈尔滨市科技创新人才专项基金项目(2006RFXXS047)
黑龙江省中医药管理局科研项目(ZHY08-W26)
关键词
脑出血大鼠模型
“百会透曲鬓”针刺法
IL-6
Rat model of intracerebral hemorrhage
Acupuncture of BaiHui - QuBin
Interleukin - 6
