期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

体外受精-胚胎移植术后早期自然流产蜕膜组织中Livin的表达 被引量：1

Study of apoptosis and the expression of Livin in decidua of spontaneous abortion after IVF-ET
原文传递
导出
摘要 目的研究体外受精-胚胎移植(IVF-ET)术后早期自然流产与正常妊娠人工流产蜕膜组织中细胞凋亡与凋亡抑制蛋白Livin的表达,分析并探讨细胞凋亡及Livin与IVF-ET术后自然流产的关系。方法对30例IVF-ET术后早期自然流产患者(研究组)和22例正常妊娠要求人工流产患者(对照组)的蜕膜组织进行研究。应用TdT介导的dUTP缺口原位末端标记技术(TUNEL)检测细胞凋亡情况;应用免疫组化SP法检测Livin的表达。结果研究组蜕膜组织细胞凋亡指数(AI)(42.07±8.26%)明显高于对照组(17.86±7.40%),差异有统计学意义(P<0.05);研究组蜕膜组织中Livin的表达(2.33±1.40分)明显低于对照组(5.64±1.94分),差异有统计学意义(P<0.05)。结论 IVF-ET术后早期自然流产患者蜕膜组织中Livin蛋白表达下降导致细胞凋亡过度,可能在自然流产的发生发展中起重要作用。 Objective：To study apoptosis and the expression of Livin in decidua of spontaneous abortion after IVF-ET,and investigate the relationship between the expression of Livin and spontaneous abortion after IVF-ET.Methods： Decidua of first-trimester were obtained from 22 surgical aspiration（the controlled team） and 30 spontaneous abortion after IVF-ET cases.Apoptosis was detected by using terminal deoxynucleotidy transferase-mediated dUTP-biotin nick ending labeling（TUNEL）;Immunohistochemistry SP method were respectively adopted to measure the expression level of decidua.Results： The apoptosis indexes（AI）（42.07±8.26%）of decidua in spontaneous abortion after IVF-ET were significantly increased in comparison with the controlled team（17.86±7.40%）（P0.05）;while the expression of Livin in spontaneous abortion after IVF-ET（2.33±1.40） was significantly lower than that of the controlled team（5.64±1.94）（P0.05）.Conclusion： The expression of Livin decreased in decidua of spontaneous abortion after IVF-ET cause the increasment of apoptosis,may play a significant role in spontaneous abortion after IVF-ET.
作者 娄华 王兴玲 孙丽君 管一春 李巍巍
机构地区 郑州大学第三附属医院生殖医学中心
出处 《中国优生与遗传杂志》 2012年第4期123-124,126,共3页 Chinese Journal of Birth Health & Heredity
关键词 体外受精-胚胎移植 自然流产 蜕膜 凋亡 LIVIN TUNEL IVF-ET Spontaneous abortion Decidua Apoptosis Livin TUNEL method
分类号 R714.21 [医药卫生—妇产科学]
  • 相关文献

参考文献9

  • 1罗淄资,姚穗.cFLIP在早期自然流产绒毛组织的表达及意义[J].现代妇产科进展,2009,18(6):424-426. 被引量：7
  • 2席娜,许良智.细胞凋亡与流产[J].四川医学,2005,26(7):803-805. 被引量：5
  • 3丁峰,傅国强,邢福祺,陈士岭,刘以训.自然流产中的早孕期蜕膜细胞Bcl-2/Bax比例异常[J].基础医学与临床,2002,22(4):363-367. 被引量：12
  • 4Minas V,Jeschke U,Kalantaridou SN,et al.Abortion is associatedwith increased expression of FasL in decidual leukocytes and apoptosisof extravillous trophoblasts:a role for CRH and urocortin[].MolHum Reprod.2007
  • 5Konno R,Yamakawa H,Utsunomiya H,et al.Expression of survivin and Bcl-2 in the normal human endometrium[].Molecular Human Reproduction.2000
  • 6Kasof GM,Gomes BC.Livin, a novel inhibitor of apoptosis protein family member[].Journal of Biological Chemistry.2001
  • 7Ka H,Hunt JS.Temporal and spatial patterns of expression of inhibitors of apoptosis in human placentas[].American Journal of Pathology.2003
  • 8Petersen J,Hagan IM S.Pombe aurora kinase /survivin is required for chromosome condensation and the sp indle checkpoint attachment response[].Current Biology.2003
  • 9Huppertz B,Herrler A.Regulation of proliferation and apoptosis during development of the preimplantation embryo and the placenta[].Birth Defects Res C Embryo Today.2005

二级参考文献29

  • 1Kokawa K,Shikone T,Nakano R,et al.Apoptosis in human chorionic villi and deciduas during normal embryonic development and spontaneous abortion in first trimester[J].Placenta,1998,19:21-26
  • 2De Falco M,Penta R,Laforgia V,et al.Apoptosis and human placenta:expression of protein belonging to different apoptosis pathway during pregnancy[J].Exp Clin Cancer Res,2005,24:25-23
  • 3Oyarzo MP,Medeiros LJ,Atwell C,et al.c-FLIP confers resistance to FAS-mediated apoptosis in anaplastic largecell lymphoma[J].Blood,2006,107:2544-2547
  • 4Rasper DM,Vaillancourt JP,Hadano S,et al.Cell death attenuation by Usurpin',a mammalian DED-caspase homologue that precludes caspase-8 recruitment and activation by the CD95 (Fas,APO-1) receptor complex[J].Cell Death Differ,1998,5:271-288
  • 5Ashkenazi A,Dixit VM.Death receptor:signaling and modulation[J].Science,1998,281:1305-1308
  • 6Harwood SM,Yaqoob MM,Allen DA.Caspase and calpain function in cell death:bridging the gap between apoptosis and necrosis[J].Ann Clin Biocbem,2005,42:415-431
  • 7Kokawa K,Shikone T,Nakano R,et al.Apoptosis in human chorionic villi and deciduas during normal embryonic development and spontaneous abortion in the first trimester[J]. Placenta,1998,19(1):21～26
  • 8Smith SC,Baker PN,Symonds EM.Plaental apoptosis in normal human pregnancy[J].Am J Obstet Gynaecol,1997,177(1):57～65
  • 9Chan CC,Lao TT,Cheung AN.Apoptotic and profliferative activities in first trimester placenta[J].Placenta ,1999,20(2～3):223～227
  • 10Dai D,Moulton BC,Ogle TF.Regression of the decidualized mesometrium and decidual cell apoptosis are associated with a shift in expression of bcl-2 family members[J].Biol Reprod,2000,63(1):188～195

共引文献19

同被引文献29

  • 1Salvesen GS, Duckett CS. IAP proteins: blocking the road to death's door[J]. Nat Rev Mol Cell Biol, 2002, 3 (6) : 401- 410.
  • 2Kasof GM, Gomes BC. Livin, a novel inhibitor of apoptosis protein family member[J]. J Biol Chem, 2001, 276 (5) : 3238-3246.
  • 3Nedelcu T, Kubista B, Koller A, et al. Livin and Bcl-2 expression in high-grade osteosarcoma[J]. J Cancer Res Clin Oncol, 2008, 134 (2) : 237-244.
  • 4Hakhyun KA, Hunt JS. Temporal and spatial patterns of expression of inhibitors of apoptosis in human placentas[J]. Am J Pathol, 2003, 163 (2) : 413-422.
  • 5Ashhab Y, Alian A, Polliack A, et al. Two splicing variants of a new inhibitor of apoptosis gene with different biogical properties and tissued distribution pattern[J]. FEBS Lett, 2001, 495 (122) : 56-60.
  • 6Nishihara H, Kizaka-Kondoh S, insel PA, et al. Inhibition of apoptosis in normal and transformed intestinal epithelial cells by cAMPthroughinductionofinhibitorofapoptosisprotein (IAP) - 2[J]. Proc NatlAcad Sci USA, 2003, 100 (15) : 8921- 8926.
  • 7Vucic D, Franklin MC, Wallweber HJ, et al. Engineering ML- IAP to produce an extraordinarily potent caspase9 inhibitor: implications for Smac-dependent anti-apoptotie activity of ML- IAP[J].BiochemJ, 2005, 385 (Ptl) : 11-20.
  • 8Ma L, Huang Y, Song Z, et al. Livin promotes Smac/DIABLO degradation by ubiquitin-proteasome pathway[J]. Cell Death Differ, 2006, 13 (12) : 2079-2088.
  • 9Sanna MG, da Silva Correia J, Ducrey O, et al. IAP suppression of apoptosis involves distinct mechanisms: The TAKI/JNK1 signaling cascade and caspase inhibition[J]. Mol Cell Biol, 2002, 22 (6) : 1754-1766.
  • 10Yuan D, Liu L, Gu D. Transcriptional regulation of Livin by beta-catenin/TCF signaling in human lung cancer cell lines[J]. Mol CellBiochem, 2007, 306 (1-2) : 171-178.

引证文献1

二级引证文献2

中国优生与遗传杂志
2012年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部