摘要
目的:探讨人工流产与自然流产蜕膜组织中凋亡调控基因的表达。方法:人工流产和反复自然流产蜕膜组织各30份,应用免疫组织化学方法测定Bc l-2、Bax、P 53、Fas、FasL 5种调控基因在蜕膜组织中的蛋白表达。结果:Bc l-2的表达在人工流产组与自然流产组无明显差异(P>0.05);Bax在自然流产组中的表达较人工流产组明显增强(P<0.05);FasL的表达在自然流产组明显低于人工流产组(P<0.05);Fas表达自然流产组与人工流产组无明显差异(P>0.05);P 53蛋白在自然流产组的表达强于人工流产组(P<0.05)。结论:自然流产的发生有3条凋亡调控途径:一为Fas/FasL途径:由于滋养细胞表面FasL表达的减少,不能引起母体内特异性的活化T细胞的凋亡,导致母体对胚胎的排斥反应,引起自然流产;另一为Bc l-2/Bax途径:虽然Bc l-2的表达在人工流产与自然流产蜕膜中无明显差异,但由于Bax在早孕自然流产蜕膜组织中的表达明显增强,导致Bc l-2/Bax的表达量失衡而引起流产;再一为P 53途径:野生型的P 53增多诱发细胞凋亡而导致流产发生。
Objective： To question the expression of apoptosis control gene in deciduas of spontaneous abortion and artificial abortion. Methods： Deciduas tissue from 15 cases of recurrent spontaneous abortion and30 cases of artificial abortion, to assay the protein expression of Bcl -2, Bax, P53, Fas, FasL in deciduas by immunohistochemistry. Results： expression of Bcl -2 has not obviously difference in spontaneous abortion group and artificial abortion group （ P 〉 0. 05 ） ; expression of Bax was obviously strong in spontaneous abortion group （ P 〈 0. 05 ） ; expression of FasL was obviously weaker in spontaneous abortion group than artificial abortion group （ P 〈 0. 05 ） ; expression of Fas has not obviously difference in spontaneous abortion group and artificial abortion group （ P 〉 0. 05 ） ; expression of P53 was obviously strong in spontaneous abortion group （ P 〈 0. 05 ） . ConclusiOn： Three apoptosis control pathway correlated to spontaneous abortion , the first is Fas/FasL pathway： the expression of FasL location in trophoblast surface decrease, dont make maternal specific activation T cell apoptosis, to lead to reject reaction and spontaneous abortion; the second is Bcl- 2/Bax pathway： although expression of Bcl -2 has not obviously difference in spontaneous abortion group and artificial abortion group, expression of Bax has obviously reinforcement in spontaneous abortion group, lead to Disequilibrium of the expression of Bcl - 2/Bax and abortion , the third is P53 pathway ： the increasing of wild type P53 induce apoptosis and spontaneous abortion.
出处
《中国妇幼保健》
CAS
北大核心
2006年第15期2132-2134,共3页
Maternal and Child Health Care of China
关键词
自然流产
蜕膜
凋亡调控基因
蛋白表达
Spontaneous abortion
Decidua
Apoptosis control gene
Protein expression
