摘要
在压电石英金电极上自组装半胱胺膜,通过1-乙基-3-(3-甲基氨丙基)碳二亚胺盐酸盐和N-羟基琥珀酰亚胺活化的羧基化碳纳米管交联半胱胺和DNA探针,构建新的压电DNA传感器,实现对肺癌特征DNA的压电检测。该传感器检测灵敏度为27.617Hz/(μg/mL),线性范围为0.02～7.50μg/mL,再生性能好。样本分析结果与聚合酶链式反应(PCR)法无显著差异,可用于临床检测。
The cysteamine film was immoboilized on a quartz crystal gold electrode＇s surface by self-assembly.DNA probes of lung cancer and cysteamine film were linked with carbonylic carbon nanotubes which been activated with 1-ethyl-3-（3-dimethyllaminopropyl） carbodiimide hydrochloride（EDC） and 1-hydroxy-2,5-pyrrolidinedione（NHS）.Thus,a new lung cancer piezoelectric DNA sensor was constructed to detect DNA labeling lung cancer,and the sensitivity was 27.617 Hz/（μg/mL）,and the linear range was from 0.02 μg/mL to 7.50 μg/mL.The DNA sensor had good regenerability,and there were not significant difference between the DNA sensor and PCR in samples detection.
出处
《压电与声光》
CSCD
北大核心
2012年第2期249-252,共4页
Piezoelectrics & Acoustooptics
关键词
肺癌
DNA传感器
压电检测
碳纳米管
lung cancer
DNA sensor
piezoelectric detection
carbon nanotubes
