摘要
背景与目的50%-80%的非小细胞肺癌存在染色体短臂3p21.3上等位基因的丢失,提示可能存在多个潜在的抑瘤基因。本研究通过大规模筛选3p21.3区相关基因在肺癌中的表达,以期找到数个肺癌相关抑瘤基因。方法应用生物信息学方法,选择出11个位于该区与肿瘤密切相关的基因,RT-PCR检测16例非小细胞肺癌及配对组织标本中11个基因的表达,并进行相关统计分析。结果在11个基因中,5个基因[RASSF1A43.8%(7/16),GNAT137.5%(6/16),SEMA3B62.5%(10/16),SEMA3F50%(8/16),Blu56.3%(9/16)]在非小细胞肺癌组织中表达下调或缺失(P<0.05);其余6个基因ZNF35、NPRL2、LTF、AXUD1、BAP1和FUS1的表达,在非小细胞肺癌及配对组织标本的表达无统计学差异。结论肺癌组织中3p21.3区有多个基因参与了肺癌的发生发展。
Background and objective The deletion of allele located at 3p21.3 region is found in 50%-80% of nonsmall cell lung cancer （NSCLC）, suggesting that this region might harbor some tumor suppressor genes （TSGs）. It is anticipated that some TSGs closely related to NSCLC carcinogenesis might be found by screening the expression of genes, located at the commonly deleted region 3p21.3, in NSCLC. Methods Semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） and related statistical analysis were applied to examine the expression status of 11 genes closely related to tumor selected from 3p21.3 by bioinformatics in 16 NSCLC biopsies. Results No expression or down-regulation of RASSFIA, GNAT1, SEMA3B, SEMA3F, Blu were observed in 43.8% （7/16）, 37.5% （6/16）, 62.5% （10/16）, 50% （8/16）, 56.3% （9/16） respectively, in NSCLC tissues （P〈0.05）. No difference was found in another six genes （ZNF35, NPRL2, LTF, AUXDI, BAP1 and FUS1） from 11 genes in NSCLC and matched tumor-adjacent normal tissues. Conclusion Several genes identified from 3p21.3 may play a certain role in NSCLC carcinogenesis.
出处
《中国肺癌杂志》
CAS
2009年第8期853-858,共6页
Chinese Journal of Lung Cancer
关键词
肺肿瘤
基因表达
抑瘤基因
Lung neoplasms
Gene expression
Tumor suppressor genes （TSGs）
