摘要
通过建立HPLC-DAD-ESI/MS/MS分析方法对不同中药生产厂家的生脉产品进行评价。对主要成分峰进行结构鉴定。基于色谱保留时间、紫外吸收光谱、相对分子质量和多级质谱等信息,鉴定分析了12批生脉散提取物中的45个共有峰。在已建立的HPLC-DAD-MS/MS分析方法基础上,进一步对生脉散2种剂型、9个品牌的商业化产品进行了质量评价。结果表明:不同厂家在产品制备环节由于采用的药材质量参差不齐,制备工艺存在差别,造成成品制剂的质量存在明显的差异。在产品制备过程中应注意控制药材质量、提高工艺流程和质量控制水平,以确保产品的药效。
A high-performance liquid chromatography-diode array detection-tandem mass spectrometry （HPLC-DAD- MS/MS） method has been firstly developed for chemical fingerprint analysis of SMS extract and rapid identification of major compounds in the fingerprints. Comparing the UV and MS spectra with those of reference compounds, 45 peaks in the fingerprints were identified. Moreover, a similarity analysis was employed to evaluate 12 samples of SMS extracts. Based on the established LC-MS method, 9 brands of SMS products including two different dosage forms were evaluated. The results showed that not only the strictly quality regulation during the preparation procedure is important, but also the quality control of Chinese medical materials is indispensable. The application of the method to the commercial products of SMS provides chemical support for the chromatographic fingerprint technology and facilitates to improve the quality control standard of this age-old TCM formula.
出处
《中国科技论文》
CAS
北大核心
2012年第3期230-235,240,共7页
China Sciencepaper
基金
国家自然科学基金资助项目(30772792)
高等学校博士学科点专项科研基金资助项目(200803160002)
关键词
中药分析
生脉散
HPLC-DAD-MS/MS
成分鉴定
analysis of traditional Chinese medicine
Sheng-Mai San
HPLC-DAD-MS/MS
constituents identification
