摘要
对12个月的茶秆竹进行细胞壁解剖特性和木质素微区分布的研究,运用光学显微镜、激光共聚焦扫描显微镜以及颜色反应鉴定木质素的存在,利用组织化学染色方法及其可见光显微分光光度计半定量测定竹材纤维、薄壁组织和导管细胞壁各微区的木质素含量。12个月时细胞壁全部木质化,木质素在各组织中均有分布,其含量因组织类型及其细胞壁微区不同而有差异。从组织化学染色及可见光吸收光谱图的吸收峰值,说明细胞壁各微区中存在愈创木基(G)和紫丁香基(S)2种木质素组成单元。竹壁径向和纤维帽不同位置的木质素含量未有明显的规律性变化。纤维次生壁具有薄厚层交替的多层结构,薄层木质素含量大于厚层。
The anatomy characteristics of cell wall and lignin distribution in different anatomical regions for 12-month old bamboo species Pseudosasa amabilis was investigated.The lignin distribution was studied by means of optical microscope and confocal laser scanning microscope and the color reaction.In addition,the distribution of lignin in different tissues（fiber,parenchyma and vessel） was investigated by visible-light microspectrophotometry coupled with the Wiesner and Mule reaction.The cell walls of different tissues were all lignified and the lignin was widely distributed in different tissues,in which there was a difference between tissues and anatomical regions in lignin content.Guaiacyl lignin and syringyl lignin unit could be found in cell wall of fiber,parenchyma and vessel through Wiesner and Mule reaction and the absorbance peak of spectrum.There was no regular variation in lignin content with different radial location of culms and different location in fiber strands.The lamellation of the fiber cell wall was generally had as alternating broad and narrow layers with alternating low and high concentration of lignin.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2012年第2期129-133,I0001,共6页
Scientia Silvae Sinicae
基金
林业公益性行业科研专项(201004005)
国际竹藤网络中心基本科研业务费专项(1632010002)
关键词
茶秆竹
细胞壁特性
木质素微区分布
可见光显微分光光谱
Pseudosasa amabilis
characteristics of cell wall
distribution of lignin
visible-light microspectrophotometry
