摘要
考察了消毒方式、外植体部位、培养基配方等因素对薄荷(Mentha haplocalyx Brip.)组织培养的影响。结果表明,外植体先用体积分数70%的乙醇处理30 s后,再用1 mg/L HgCl2+2滴吐温80浸泡一定时间,消毒效果好于40 mg/L的漂白粉处理,适宜的HgCl2消毒时间分别为茎段、叶片、叶柄和已萌发的芽12 min;末萌发的芽10 min。茎段的不定芽诱导率和不定芽生长情况好于其他外植体。在薄荷旺盛生长期采集半木质化的茎段作为外植体进行诱导培养,不定芽萌芽早而且生长速度快。MS+0.50 mg/LBA+0.10 mg/L NAA是适合外植体不定芽诱导的培养基配方。
The effects of sterilizing methods,explant material,culture medium formula on tissue culture of mint（Mentha haplocalyx Brip.） was studied.The results showed that after explants being immersed in 70% ethanol,1 mg/L HgCl2＋Tween 80 had better disinfection effect than 40 mg/L bleaching power.The treatment time of HgCl2 for steam,leaf,leafstalk and geminated bus should be 12 min;10 min for steam,especially steam collected in the vigorous growth period of mint as explants had higher inducing rate than other explants.MS＋0.50 mg/L BA＋0.10 mg/L NAA was the suitable medium for buds induction of mint explants.
出处
《湖北农业科学》
北大核心
2012年第7期1471-1473,1480,共4页
Hubei Agricultural Sciences
基金
安徽省教育厅重点科研项目(KJ2009A030)
阜阳师范学院省级科研机构委托专项(2011KSLZX05YB)
