摘要
本文建立了一种果蔬中吡虫啉农药残留的高效液相色谱检测方法。样品经乙腈提取、盐析、LC-NH2小柱净化，采用高效液相色触紫外检测器进行测定，色谱柱：Waters Symmetry Shieldr^TM C18（4．6×250mm，5μm）；柱温：30．0℃；检测波长：275nm；流动相：甲醇：水：乙腈=55：25：20（体积比）；流速：0．8mL／min；进样量：10．0μL。吡虫啉在0．1mg／L至1．0mg／L的添加范围内线陛良好，线性关系系数r为0．9925；添加水平为0．05-0．20mg/kg时，西红柿样品的平均回收率为95．4％-106．8％，相对标准偏差为2．66％。7．99％。结果表明该方法具有快速、灵敏、准确等优点，适合蔬菜中吡虫啉农药残留的测定。
A novel high performance liquid chromatography （HPLC） method was developed for the analysis of irnidaeloprid pesticide residue in fresh vegetables. Imidaeloprid was extracted from the samples by acetouitrile, purified with LC-NH2 column and determined by high performance liquid chromatography with UV detection. Symmetry ShieldTM C18 column （4.6×250 mm, 5 pan） using the mixture of methanol, water and acetonitrile （55：25：20） as tbe mobile phase. The column temperature, flow rate, detection wavelength and sample volume were 30.0 ℃, 0.8 rnL/min, 275 nm and 10.0 μL, respectively. Within the linear range of 0.1-1.0 mg/L, the correlation coefficient, the recoveries of irnidaeloprid in tomato and the relative standard deviations （RSD） were 0.9925, 95.4%-106.8 % and 2.66%-7.99%, respectively, The method was simple, effective and accurate. It can be applied to determinate irrtidaeloprid pesticide residue in fresh vegetables.
出处
《现代食品科技》
EI
CAS
北大核心
2012年第4期466-468,482,共4页
Modern Food Science and Technology
基金
云南省科技计划项目(2009ZC098)
