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紫杉醇抗喉癌细胞作用及机制的实验研究

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摘要 目的研究紫杉醇(Taxol)抗人喉鳞状细胞癌细胞株Hep-2的作用及机制。方法以不同浓度Taxol作用于体外培养的人喉鳞状细胞癌细胞株Hep-2,用MTT法测定Taxol对细胞的增殖抑制率,分光光度法测定癌细胞膜唾液酸含量、膜磷脂含量及胆固醇含量变化。结果发现紫杉醇对人喉鳞状细胞癌细胞株Hep-2生长有明显抑制作用,且抑制作用呈浓度依赖性。癌细胞膜分析显示紫杉醇与细胞接触24 h,可显著提高膜磷脂含量,降低细胞膜唾液酸含量,但是对胆固醇含量没有影响。结论紫杉醇明显抑制人喉鳞状细胞癌细胞株Hep-2增殖,其作用机制可能与膜生化特性改变有关。
作者 马永恒 袁逸铭
机构地区 兰州大学第二医院
出处 《卫生职业教育》 2012年第7期112-113,共2页 HEALTH VOCATIONAL EDUCATION
关键词 紫杉醇 人喉鳞状细胞癌 细胞增殖
分类号 R979.1 [医药卫生—药品]
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参考文献9

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卫生职业教育
2012年 第7期

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