摘要
目的:研究ROCK2在宫颈上皮内瘤样病变(cervical intraepithelial neoplasia,CIN)及早期宫颈癌组织中的表达,探讨其与CIN疾病及早期宫颈癌进展的相关性。方法:应用免疫组化S-P法,检测30例正常宫颈、30例不同级别CIN、30例宫颈癌组织中ROCK2蛋白的表达情况。分析其表达结果与临床病理特征的关系。结果:①ROCK2蛋白在正常宫颈组织中的表达率为0%,在CIN病变组织中的表达率为30.0%,在早期宫颈癌组织中的表达率为76.7%,在官颈癌组织中的表达水平,高于CIN及正常官颈组织(P<0.01),随着从正常宫颈组织→CIN病变组织→宫颈癌的转化,阳性表达率呈递增趋势。②ROCK2的表达与病理组织分级、有无淋巴结转移相关(P<0.05),与官颈癌患者的年龄、临床分期则无明显相关关系(P>0.05)。结论:检测ROCK2对临床CIN及宫颈癌的预后,可供作有效用的一项参数。
Objective To investigate the expression of ROCK2 in CIN and cervical squamous carcinoma and its correlation with invasiveness. Methods The expression of ROCK2 was examined respectively by immunohistochemical S-P method in 30 cases of nor- real cervical tissue, 30 cases of cervical intraepithelial neoplasia and 30 cases of early-stage cervical cancer. This study analyzed staffs- tically the correlation between the immunohistochemical results and the clinicopathological features. Result ①The expression rate of ROCK2 was 0% in normal cervical tissues, 30.0% in cervical intraepithelial neoplasia and 76.7% in cervical squamous carcinoma re- spectively. The positive rate was significantly higher in the cervical cancer group than in the normal control group （ P 〈 0.01 ）. Fromnormal cervical tissue, cervical intraepithelial neoplasia（CIN） to cervical cancer, the positive expression rates of ROCK,？, were in- creased gradually. ② ROCK2 protein expression was correlated with histological grade and lymph node metastasis （P 〈 0.05 ）, but not with age and clinical stage （P 〉 0.05）. Conclusion Expression of ROCK2 may serve as an effective parameter for evaluating CIN and prognosis in cervical squamous carcinoma clinically.
出处
《放射免疫学杂志》
CAS
2012年第2期170-172,共3页
Journal of Radioimmanology
基金
南京医科大学科技发展基金项上项目(08NMUM081)
关键词
宫颈上皮内瘤样病变
早期宫颈癌
蛋白激酶
免疫组化
cervical intraepithelial neoplasma （CIN）, cervical squamous carcinomas, ROCK, immunohistochemistry
