摘要
采用集落形成试验,测定五种人肝癌细胞系的药物敏感性。结果表明,这些细胞系对药物明显抗拒,对ADM、5-Fu、DDP和MEL的敏感性依次降低。各系细胞对PYM均较敏感。与ADM显然不同,PYM对慢增殖细胞具有更强的杀伤作用。该组细胞系似可用于抗肝癌药物的筛选。
The in vitro chemosensitivities of five cell lines derived fromprimary hepatocellular carcinomas of Chinese patients were detemined. Cellsurvival was measured by colony-forming ability in an agarose system. Ifa≥50% survival reduction after a 1 h exposure to 0. 1 PPC (peak plasmaconcentration) of the drug is used as a definition of a response in vitro,only2of these cell lines are sensitive to ADM. When then concentration wasincreased to 0 .5 PPC, 2/5 cell lines were moderately sensitive to 5 Fu orDDP. In the majority of cases, a marked decrease in survival was observedwhen the duration of drug exposure was increased from 1 h to 14-16 daysin continuation whilst little effect of exposure time was seen for HMM,BCNU, MEL and TSPA. Pingyangmycin (PYM), one of the components of bleomycin (BLM),was found to be clinically effective against hepatoma. In the present study,a 1 h exposure of PYM at 0 .1μg/ml, eguivalent to 0 .025-0 .05 PPC ofBLM, produced more than 50% survival reduction in all lines. In contrastwith ADM, PYM was more effective in reducing the colony-forming abi-lity of cells in plateau-phase than those in logarithmic growth, with ID90values differing by a factor of 3 .7, being 0 .168 and 0 .625μg/ml respective-ly. On the basis of the present study, we feel that these human hepatomacell lines may retain their original resistance to the drug and could beused as an in vitro screening model fo represent particular tumor type.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
1990年第2期76-81,共6页
The Chinese Journal of Clinical Pharmacology
基金
卫生部科研基金
关键词
肝癌
人癌细胞系
药物敏感
hehatoma
human cancer cell lines
anticancer drugs
drug sensitivity
