摘要
目的探讨转化生长因子-β(TGF-β)在放射性肺损伤大鼠肺组织中的表达及意义。方法选择无特定病原体(SPF)级健康Wistar大鼠35只,随机分为对照组(5只)、照射组(30只,1d,1、2、4、8、12周各5只)。在照射1d,1、2、4、8、12周获取标本,采用免疫组化法观察放射性肺损伤大鼠肺组织细胞TGF-β水平变化。结果照射组TGF-β在肺成纤维细胞中的表达高于对照组,差异有统计学意义(P=0.000),其损伤程度随时间的推移而增加。结论 TGF-β是肺成纤维细胞(FB)活化的最重要生物介质之一,阻断TGF-β通路及FB的活化,可能为减少照射后肺间质炎症和纤维化的程度带来新的希望。
Objective To investigate the expression of TGB-β on lung tissue of rats with radiation induced lung injury.Methods Thirty-five male Wistar rats were randomly divided into 7 groups（n=5）：control group and treatment group（received irradiation on the the right hemithorax or sham right lung irradiation with single 30Gy,1day,1,2,4,8,and 12 weeks）.The rats were sacrificed at 1 day,1,2,4,8,and 12 weeks after the irradiation,and lung tissues were separated.The expression of TGF-β was detected by immunohistochemistry.Results The expression of TGF-β in fibroblast（FB） significantly increased after irradiation,compared with control group（P=0.000）.The degree of injury increased with the time passing.Conclusion TGF-β was an important biological mediator during the activation of fibroblast.We suspected that FB might be the target cell for prevention and cure the radiation induced lung injury.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第10期964-965,F0003,共3页
Chongqing medicine
基金
广东省自然科学基金资助项目(S2011040003476
8451051501001344)
广东省医学科研基金资助项目(A2010367)
关键词
放射性肺损伤
成纤维细胞
转化生长因子-Β
radiation-induced pulmonary injury
fibroblast
transforming growth factor-β
