摘要
目的了解该院女职工人乳头瘤病毒(HPV)感染情况。方法采用聚合酶链反应(PCR)和DNA反向点杂交相结合的DNA芯片技术对该院670例女职工进行HPV感染及HPV亚型检测。结果 HPV感染率为12.39%(83/670),其中单一、双重和三重及其以上基因型感染者分别占75.90%(63/83)、14.46%(12/83)和9.64%(8/83);单纯低危型和单纯高危型感染率分别为10.84%(9/83)和78.31%(65/83),低危型与高危型混合感染率为10.84%(9/83)。共检出19种基因型,以高危型HPV52检出率最高[2.84%(19/670)]。结论该院女职工HPV感染率较高,多重感染及高危型感染情况较为严重,应重视HPV筛查在疾病预防中的重要作用。
Objective To understand the prevalence of human papillomavirus（HPV） infection in female medical workers in this hospital.Methods Gene chip technique was performed for the detection of HPV infection and HPV genotypes in 670 cases of female medical workers.Results HPV infection rate was 12.39%（83/670）,and single,double and treble genotypes infection were 75.90%（63/83）,14.46%（12/83） and 9.64%（8/83）respectively among all positive cases.Single low-risk and single high-risk genotype infection rates were 10.84%（9/83） and 78.31%（65/83）,Nineteen genotypes could be demonstrated and the most predominant subtype was HPV52[2.84%（19/670）].Conclusion HPV infection rate might be relatively high in female medical workers in this hospital,and the prevalence of multiple infection and high-risk genotype infection was severe.HPV screening should be performed for the prevention of relative diseases.
出处
《国际检验医学杂志》
CAS
2012年第6期689-690,692,共3页
International Journal of Laboratory Medicine
关键词
宫颈肿瘤
人乳头瘤病毒
基因亚型
宫颈癌
DNA芯片
uterine cervical neoplasms
human papillomavirus
gene subtypes
cervial carrcinoma
DNA chip
