摘要
目的 :介绍成人包皮角朊细胞的分离、培养、传代、冻存、复苏及鉴定技术。方法 :采用细胞工程技术及免疫组化技术。结果 :采用细胞工程技术至少可将角朊细胞传至 6代以上 ,此时的细胞数至少为原代培养的细胞数的 1万倍 ,经冻存、复苏后 ,此角朊细胞仍既能增殖、又能分化 ,生长状态与原代培养的角朊细胞相类似 ;免疫组化显示 :AE1反应阳性。结论
? Objective:To establish human keratinocyte bank in vitro.Methods:Cell engineering technique was used.The techniques of isolation,culture,subculture,frozen storage,recovery and identification of adult human keratinocytes were introduced in this paper.Results:The keratinocytes were passed atleast6 passages with more than 1 0 0 0 0 times the original plating number.After frozen storage and recovery,the keratinocytes could proliferate and differentiate,which showed similar growth conditions as primarily cultured keratinocytes. Conclusion:The keratinocytes bank was established. This technique for establishment of human keratinocytes bank was easy,simple and feasible under routine laboratory conditions. 〔
出处
《白求恩医科大学学报》
CSCD
2000年第3期231-234,共4页
Journal of Norman Bethune University of Medical Science
基金
卫生部科研基金项目(96-2-178)
关键词
角朊细胞
细胞工程
细胞库
keratinocytes
cell engineering
cell bank
