摘要
目的 :分析当归多糖AP Ⅰ ,AP Ⅱ ,AP Ⅲ ,AP Ⅳ各组分的重均相对分子质量分布及其电荷特性 ;探讨多糖类生物大分子的组效关系研究方法。方法 :采用高效凝胶过滤色谱法、阴离子交换色谱法、二极管阵列检测器进行检测 ;改良苯酚 硫酸法测定糖分部 ;ServaBlue G染料结合法测定蛋白质分部。结果 :当归多糖AP Ⅰ ,AP Ⅱ ,AP Ⅲ ,AP Ⅳ分别由 4～ 5个具有不同重均相对分子质量分布及电荷特性的多糖组分组成 ;4种多糖中还分别含有一定量的游离或结合蛋白质。结论 :当归多糖AP Ⅰ ,AP Ⅱ ,AP Ⅲ ,AP Ⅳ为组成成分不同的多糖 ;该方法可为多糖组效关系研究提供简便、实用的分析方法。
OBJECTIVE:To analyze the distribution of weight average molecular weight (r) of four Angelica polysaccharides isolated from Angelica sinensis (Oliv.)Diels:AP Ⅰ,AP Ⅱ,AP Ⅲ,AP Ⅳ and determine their characters of electric charge,in order to provide a practical analytical method for studying the constituent activity relationship of polysaccharides.METHODS:Two kinds of high performance liquid chromatography methods were employed,including high performance gel filtration chromatography for r determination and high performance anion exchange chromatography for electric charge analysis.Improved phenol sulfuric acid method combined with ELISA reader were used for the polysaccharide fractions determination,and Sever blue G dye combined method for protein fractions as well.RESULTS:Each of the four Angelica polysaccharides,AP Ⅰ,AP Ⅱ,AP Ⅲ and AP Ⅳ,was composed of 4～5 polysaccharide constituents with different r distribution and characters of electric charge;and there were small amount of different free or combined proteins in the four Angelica polysaccharides.CONCLUSION:The four Angelica polysaccharides,AP Ⅰ,AP Ⅱ,AP Ⅲ and AP Ⅳ,are different from each other in r and character of electric charge;this method is practical and reliable for the study of constituent activity relationship of polysaccharides.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2000年第5期332-335,共4页
Chinese Pharmaceutical Journal
基金
Hong Kong Research Grant Council资助
关键词
当归多糖
组效关系
高效液相色谱法
分子质量
电
Angelica sinensis (Oliv.)Diels,polysaccharides,HPLC,constituent activity relationship,weight average molecular weight,electric change
