摘要
目的 观察mIL 12基因转染的小鼠树突状细胞(DC)功能的改变。方法 分离小鼠骨髓单个核细胞与rmGM CSF和rmIL 4培养 1周 ,对培养的细胞进行形态学观察 ,FACS检测细胞表面DEC2 0 5、CD86表达。以mIL 12重组腺病毒转染培养的DC ,ELISA测定培养上清中mIL 12的水平 ,混合淋巴细胞反应检测转染细胞的功能。结果 培养 1周后 ,得到具有典型DC形态的细胞 ,以mIL 12重组腺病毒为载体转染DC ,培养上清中可以检测到较高水平的mIL 12 ;与对照组相比转染细胞能明显地刺激混合林巴细胞反应。结论 小鼠骨髓单个核细胞经GM CSF和IL 4培养 1周 ,生成大量DC ,mIL
Objective To observe the function of dendritic cells(DC) transfected with mIL 12 (murine IL 12) gene. Methods Monocytes isolated from murine bone marrow were cultured with rmGM CSF and rm IL4 for 7 days. Characteristics of the cultured cells were observed and the expression of DEC205 and CD86 was detected by FACS. DC derived from culture were transfected with recombinant adenovirus containing mIL 12 gene. mIL 12 in the supernatant was detected by ELISA. MLR was studied with different types of DC. Results After 7 days of culture, a large number of cells with typical characteristics of DC were observed. The mIL 12 level in the supernatant of transfected DC was high. More noticeable MLR was found with DC transfected mIL 12 gene in comparison with the control. Conclusion Generatin of large number of DC from murine bone marrow monocyte cultures supplemented with GM CSF and IL 4 for 1 week. The function of DC transfected with AdCMIVIL 12 enhanced in mixed lymphocyte reaction.
出处
《安徽医科大学学报》
CAS
2000年第2期94-97,共4页
Acta Universitatis Medicinalis Anhui
