摘要
目的：观察 ｍ Ｉ Ｌ１２ 基因转染的小鼠 Ｅ Ｌ４ 细胞（ Ｃ５７ Ｂ Ｌ／６ 小鼠 Ｔ 淋巴瘤细胞株）体外生物学特性的改变。方法：阳离子脂质体协同 Ｉ Ｌ１２ 重组腺病毒感染 Ｅ Ｌ４ 细胞。结果及结论：基因转染的 Ｅ Ｌ４ 细胞有 ｍ Ｉ Ｌ１２ 的 ｍ Ｒ Ｎ Ａ 表达，培养上清中可以检测到 ｍ Ｉ Ｌ１２ 的生物学活性。与野生型 Ｅ Ｌ４ 细胞相比，ｍ Ｉ Ｌ１２ 重组腺病毒感染的 Ｅ Ｌ４ 细胞形态及体外增殖能力无明显改变， 转染细胞的成瘤性降低。此外，研究结果还表明，阳离子脂质体能显著提高腺病毒的感染效率。
Objective: To observe the biological characteristics of EL 4 cells transfected by murine interleukin 12 gene. Methods: Adenoviral vector containing mIL 12 was used to infect EL 4 cell line with the adjutant of lipofectamine. Results and Conclusion: mIL 12 Could be detected in the culture supernatant by ELISA. IL 12 mRNA Transcription could also be detected by RT PCR. In comparison with wild type EL 4 cells, mIL 12 modified EL 4 cells have no difference in growth. The tumorigenicity of mIL 12 modified cells was decreased. It was also found that lipofectamine could increase the efficiency of transfection by adenoviral vector.
出处
《军事医学科学院院刊》
CSCD
北大核心
1999年第3期193-196,共4页
Bulletin of the Academy of Military Medical Sciences
关键词
白细胞介素12
EL-4细胞
腺病毒感染
基因转染
murine interleukin 12
EL 4 cell line
adenovirus infection
lipofectamine
transfection
