摘要
目的建立测定四乙酰葛根素的含量及有关物质的超高效液相色谱法。方法采用Agilent XDB-C18色谱柱（4.6mm×250mm,5μm）,流动相：乙腈-水（45∶55）,流速：1.2 mL.min-1,检测波长：250nm,柱温：30℃。结果四乙酰葛根素的浓度在0.020 76～0.186 8mg.mL-1与峰面积线性关系良好（A=1.287×104 C-24.55,r=0.999 9,n=7）,检测限为6.65ng.mL-1（S/N=3）;平均加样回收率为99.9%（RSD=1.4%,n=9）;有关物质含量为1.02%～1.28%。结论该方法简便、准确,可用于四乙酰葛根素的含量及有关物质的测定。
Objective To establish a UPLC method to determine the content and related substances of tetra-acetylated puerarin.Methods Agilent XDB-C18 chromatographic column（4.6 mm×250 mm,5 μm）was used and the mobile phase consisted of acetonitrile-water（45∶55）with a flow rate of 1.2 mL·min-1.The UV detection wavelength was 250 nm.The column temperature was 30 ℃.Results The calibration curve was linear over 0.020 76-0.186 8 mg·mL-1（A=1.287×104C-24.55,r=0.999 9,n=7）.The detection limit was 6.65 ng·mL-1（S/N=3）.The average recovery was 99.9%（RSD=1.4%,n=9）.The content of related substances of tetra-acetylated puerarin was over 1.02%-1.28%.Conclusion The method is simple,accurate,and applicable for the quality control of tetra-acetylated puerarin.
出处
《中南药学》
CAS
2012年第3期196-198,共3页
Central South Pharmacy
基金
国家科技部重大专项项目（No.2009ZX09103-150）
