摘要
目的了解山东大学家蝇抗药性现状,预测抗性发展趋势。方法生物测定法与分子检测法相结合。结果生物测定结果表明,山东大学家蝇对常用的拟除虫菊酯类杀虫剂的抗性已经达到中、高度水平;对山东大学家蝇个体进行钠离子通道突变位点检测,发现抗性个体占种群的80.43%,然而纯合子仅为28.26%。结论将两种测定方法结果结合,清楚地表明虽然山东大学家蝇对拟除虫菊酯类杀虫剂已经达到中、高度抗性,但种群内仍有大部分为杂合子,应及时采用合理措施,延缓家蝇抗药性的发展。
Objective To investigate the resistance of Musca domestica in Jinan city and forecast its development trends.Methods Combination of the dipping method and molecular detection.Results Resistance of M.domestica in Jinan city had developed to middle or high levels;the results obtained by molecular detection showed that resistant individual numbers made up 80.43% in total individual numbers,but resistant homozygous was only 28.26%.Conclusion Resistance of M.domestica in Jinan city to pyrethrin chemicals has developed to middle or high levels,however,most heterozygous still exist in population,indicating that reasonable management should be recommended for delaying the development of M.domestica resistance.
出处
《中华卫生杀虫药械》
CAS
2012年第2期96-98,101,共4页
Chinese Journal of Hygienic Insecticides and Equipments
基金
山东大学科技发展基金项目(编号:2006-80)
关键词
家蝇
抗药性
钠离子通道
基因突变
Musca domestica
resistance
the sodium channel
gene mutation
