摘要
目的 :建立反相高效液相色谱法测定滇桂艾纳香中原儿茶酸的方法。方法 :滇桂艾纳香水提液醋酸乙酯 乙醇 (4∶1)提取 ,薄层色谱分离 ,反相高效液相色谱法测定 ,μbondapakC18色谱柱 ,流动相甲醇 水 冰醋酸 (19∶80∶1) ,检测波长 2 6 0nm。结果 :进样量 3.31～ 41.8μg·ml-1,线性关系良好 (r =0 .9999) ,加样回收率为 98.0 5 % ,RSD=1.94% (n =6 )。结论
Objective:To determine the content of protocatechuic acid in Blumea riparia by RP HPLC.Method:μ Bondapak C 18 column was used,mobile phase consisted of methanol water glacial acetic acid(19∶80∶1) and detection was performed at UV 260 nm.Result:The standard curve was linear in the range of 3.31～41.8 μg·ml -1 .The correlation coefficient was 0.9999.The average recovery rate and RSD were 98.05% and 1.94%( n =6) respectively.Conclusion:The method provides scientific indexes for quality control of riparia.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2000年第4期227-229,共3页
China Journal of Chinese Materia Medica
