摘要
目的：建立脑心清分散片中原儿茶酸的高效液相色谱测定方法。方法：采用高效液相色谱法测定，C18色谱柱（200mm×4．6mm，5μm），甲醇-水（10：90）（用冰醋酸调节pH值至2．8）为流动相，检测波长256mm，柱温为室温，进样量为10μl。结果：原儿茶酸在30-600ng范围内线性关系良好，r=0．0990（n=5）。平均回收率为98．52％（n=5）。结论：本测定方法为脑心清分散片的质量评价提供了可靠的依据。
Objective： To establish the method of HPLC for determination of the dihydroxy benzoic acid in Naoxinqing dispersible tablet. Method： The HPLC was used as the assay method, the system consisted of Cls （ 200mm × 4.6mm,5 μm） column, mobile phase of menthol - water（ 10 ： 90）, the detection wavelength at 256 nm. the column temperature was room temperature, with sample size 10μL. Result： Dihydroxy benzoic acid has a good linearity at the range of 30 -600 ng, r =0.9999（n =5）. The average recovery was 98.52% （n =5）. Conclusion： the method supply a reliable basis for quality evaluation of naoxinqing dispersible tablet.
出处
《中医药导报》
2006年第7期84-85,89,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
