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血管紧张素(1-7)与血管紧张素Ⅱ对THP-1巨噬细胞高密度脂蛋白受体表达的影响 被引量：1

Effect of angiotensin Ⅰ-Ⅶ and angiotensin Ⅱ on expression of high density lipoprotein receptor in THP-1 macrophages
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摘要 目的观察血管肾张素（1-7）[Ang（1-7）]及AngⅡ对THP-1巨噬细胞高密度脂蛋白受体Ⅰ（SR—B1）表达的影响。方矬将THP—1巨噬细胞根据AngⅡ和Ang（1-7）对SR—B1表达的影响分别分为：空白对照组及不同浓度AngⅡ组（10～10^4nmol／L组）；空白对照组及不同浓度Ang（1-7）组（10-10^4nmol／L组）；空白对照组、AngⅡ10^2nmol／L组、AngⅡ10^2nmol／L＋Ang（1-7）组（10^2～10^4nmol／I，组）、AngⅡ＋Ang（1-7）＋A-779组。运用RTPCR和Western blot法检测各组sR-BImRNA及SR—BI蛋白表达的变化。结果与空白对照组比较，AngⅡ10～10^4nmol／L组SR～BImRNA及蛋白表达明显下调，呈浓度依赖性（P〈0．05）；而Ang（1—7）10～10^4nmol／L组SRBImRNA及蛋白表达明显上调，呈浓度依赖性（P〈0．05）；与空白对照组和AngⅡ组比较，AngⅡ10^2nmol／L＋Ang（17）组（10^2～10^4nmol／L组）SR-BI表达明显上调，呈浓度依赖性（P〈0．05）。与空白对照组比较，AngⅡ＋Ang（1—7）10^2nmol／L＋A-779组sR—BImRNA及蛋白表达明显下调（P〈0．05）。结论Ang（1～7）通过其特异性MAs受体浓度依赖性地拮抗AngⅡ抑制SR—BI的表达，促进胆固醇外流，提高了胆固醇逆转运的效率。 Objective To observe the effect of angiotensin Ⅰ -Ⅶ and angiotensinⅡ on expression of high density lipoprotein receptor I（SR-BI） in THP-1 macrophages. Methods THP-1 macrophages were divided into blank control group, angiotensinⅡ group, angiotensinⅡ＋ angiotensinⅠ -Ⅶ group, angiotensln Ⅱ ＋angiotensin Ⅰ -Ⅶ ＋A-779 group according to their effect on expression of SR-BI in THP-1 macrophages. Expression of SR-BI mRNA and protein in THP-1 macrophages was detected by RT-PCR and Western blot,respectively. Results The expression level of SR-BI mRNA and protein in THP-1 macrophages was significantly lower in 10 nmol/L-104 nmol/L angiotensin Ⅱ group and 102 nmol angiotensin Ⅱ＋ A-779 group than in blank control group and significantly higher in 10 nmol/L-104 nmol/L angiotensinⅠ -Ⅶ group and angiotensin Ⅱ ＋102 nmol/L-104 nmol/L angiotensinⅠ -Ⅶ group than in angiotensin Ⅱ group and blank control group（P 〈 0. 05）. Conclusion Angiotensin Ⅱ down-regulates the expression of SR-BI in a dose-dependent manner and promotes the outflow of cholesterol, thus improving its inverted tranport.
作者 柴婵娟 杨志明 李芳 梁斌 边云飞 康玉明
机构地区 山西医科大学第二医院心内科 西安交通大学医学院生理教研室
出处 《中华老年心脑血管病杂志》 CAS 北大核心 2012年第4期417-420,共4页 Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金 山西省科技攻关项目(20100311098-4)
关键词 血管紧张素类 血管紧张素Ⅱ 巨噬细胞 脂蛋白类 HDL 逆转录聚合酶链反应 RNA 信使 angiotensins angiotensin Ⅱ macrophages lipoproteins, HDL reverse transcriptase polymerase chain reaction RNA, messenger
分类号 R363 [医药卫生—病理学]
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参考文献7

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二级参考文献19

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共引文献16

同被引文献12

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中华老年心脑血管病杂志
2012年 第4期

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