摘要
B-LB基因编码的鸡MHC(major histocompatibility complex)Ⅱ类分子β链在MHCⅡ类分子递呈抗原中起重要作用。应用RT-PCR方法从鸡淋巴细胞中扩增了全长B-LB基因,其大小为792 bp。进一步经脂质体法,将其分别转染COS7和P815。B-LB基因表达在COS7的浆膜。经G418的筛选(0.6 mg/mL)得到稳定表达的B-LB的细胞株P815(B-LB)。将其培养10代次后,在RT-PCR中能获得相应DNA片段。上述结果表明B-LB基因能够在动物真核细胞中表达,并成功建立了能够稳定表达B-LB基因的P815(B-LB)细胞株,为进一步研究MHCⅡ类分子β链在免疫应答中的作用奠定了基础。
：B-LB gene encodes the β chain of chicken MHC （major histocompatibility complex） calss II molecule, which plays an important role in antigens presentation. The full-length B-LB gene with a length of 792 bp was amplified by RT-PCR method from chicken lymphoeytes. Then this segment was transfected into COS7 and P815 cells with Lipo- feetin reagent respectively. B-LB gene could be expressed on palsma membrane of COS＇/cells ; A cell strain of stable ex- pression of B-LB gene, P815-B-LB, was obtained by adding G418 （0.6 mg/mL）. After the cell passed for 10 times the specific B-LB segment could be amplified by RT-PCR. All the results showed that a cell strain of P815 （B-LB） with stable expression of B-LB was successfully established, which proved a foundation for further researching the effects of MHC II class molecule β chain in immune response.
出处
《激光生物学报》
CAS
CSCD
2012年第1期8-11,共4页
Acta Laser Biology Sinica
基金
国家自然科学基金(No.31172306)
