摘要
本文由ELISA、亲和层析和PCA等实验方法证明氨苄青霉素抗血清中存在着多种抗原结合部位特异性不同的氨苄青霉素抗体。不同氨苄青霉素抗体的组合及含量的变化,决定了抗血清的表现特异性,使之表现出极大的不均一性;用DEAE葡聚糖凝胶分级分离抗血清,发现氨苄青霉素抗体的特异性和其所带电荷有关;氨苄青霉素抗体和结构不同的过敏原之间的匹配程度,直接影响着过敏反应发生的强弱。
Using ELISA, affinity chromatogaphy and PCA etc immunological techniques, several kinds of anti-ampicillin antibodies, which had a slightly different specificity of antigen combining region of antibody, were found from the sera immunized by ampicillin preparations. Also the anti-penicillin serum could be divided into different fractions with a different charges by ion-exchange chromatography on DEAE-sephadex A-25. Each fraction had a diffeifent characteristic in antigen-antibody interaction. The variety of composition and content of anti-ampicillin antibody determined the specificity of a serum and made the sera had a very distinct heterogeneity.All kinds of anti-ampicillin antibodies were grouped under two categories PBO-ph-ile antibody with a high affinity to BPO antigenic determinant and poly-ampicillin-ph-ile antibody with a high affinity to ampicillin polymer, by PCA reaction. The interaction between the antibodies of different specificity and the antigens of different structure influenced upon the intensity of aaaphylaxis of ampicillin.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
1990年第2期108-112,共5页
Chinese Journal of Antibiotics
关键词
氨苄青霉素
抗体
过敏反应
不均性
Aapicillin
Heterogeneity of antibody
Anaphylaxis
Antigen-antibody interaction
Affinity Chromatography
