摘要
采用超临界流体萃取(SFE)技术和CGC技术测定了中药补骨脂中的补骨脂素和异补骨脂素。对超临界流体萃取过程中影响萃取效率的主要因素采用正交设计法和方差分析法进行了考察,确定了主效应和适宜的操作条件。与传统的萃取法比较,SFE具有经济、快速、简便、选择性好、环境污染小等优点。
A method based on SFE and CGC has been developed for qualitative and quantitative analysis of major components in Psoralea corylifolia L The orthogonal design method and the analysis of variance are used to optimize the five variables (temperature , pressure, volume of modifier, static extraction time and CO 2 dynamic extracting volume ) of SFE and the optimized conditions listed as follows: temperature 70 ℃, pressure 38.5 MPa, static extraction 1 min, dynamic extraction 7 mL and 60 μL CHCl 3 as modifier. The recoveries of psoralen and isopsoralen are 96.93% (RSD 1.87%) and 98.43% (RSD 2.06%) respectively. Being compared with conventional methods, the SFE as an alternative technique for sample preparation will be most useful in the quality control of Chinese traditional medicines.
出处
《色谱》
CAS
CSCD
北大核心
2000年第1期61-63,共3页
Chinese Journal of Chromatography
基金
国家自然科学基金!资助项目 (3 9770 90 4 )
关键词
超临界流体萃
补骨脂
补骨脂素
异补骨脂素
supercritical fluid extraction
capillary gas chromatography
orthogonal design
Psoralea corylifolia L.
psoralen
isopsoralen
