摘要
用反相高效液相色谱法(HPLC)和紫外分光光度法(UV)测定了β环糊精(βCD)与维生素D2(VD2)包合物中VD2的质量分数,两种方法测得的结果十分吻合。研究了HPLC测定的精密度和回收率,其相对标准偏差分别为0.63%和1.54%,表明该法准确可靠。含有βCDVD2包合物的“龙牡壮骨冲剂”经KOH、维生素C和乙醇水(体积比为1∶2)于70℃下皂化处理,再由HPLC检测用乙醚萃取的VD2产物,得到准确的结果。
In order to enhance the stability of vitamin D 2(VD 2), the inclusion complex of VD 2 with β cyc lodextrin(β CD)was added in “Longmu Zhuanggu Chongji”, a medicine of children for the prevention and cure of rickets. The VD 2 in β CD VD 2 inclusion complex was satisfactorily determined by both reversed phase high performance liquid chromatography(HPLC)and ultraviolet spectrometry(UV). The experimental results from UV were in well consistent with those from HPLC. The accuracy and recovery of HPLC measurement were analyzed, and their relative standard deviation(RSD)were given to be 0.63% and 1.54%, indicating the method is quite correct and reliable, and the linear regression equation is reasonable. The “Longmu Zhuanggu Chongji” containing β CD VD 2 inclusion complexes was saponified by a mixture of KOH, vitamin C(VC)and alcohol water(1∶2,V/V)at 70 ℃,then extracted with petroleum ether.An accurate content of the VD 2 product was obtained by HPLC. A simple and rapid HPLC method, which can detect VD 2 in the inclusion complex and in “Longmu Zhuanggu Chongji”, has been established in this work.
出处
《色谱》
CAS
CSCD
北大核心
2000年第1期52-54,共3页
Chinese Journal of Chromatography
基金
武汉健民制药厂委托科研项目
关键词
维生素D
Β-环糊精
包合物
紫外光谱
HPLC
high performance liquid chromatography
vitamin D 2
β cyclodextrin
inclusion complex
ultraviolet spectroscopy
