摘要
目的:寻找醛固酮独立致心肌纤维化(MF)的分子。方法:利用差速贴壁法分离培养胎儿心肌成纤维细胞(FCFs);选择3 4代细胞给予醛固酮(ALD)处理8 h、提取各组总RNA,用基因组芯片检测差异表达基因,利用Westernblot和RT-PCR对部分基因表达产物进行验证。结果:ALD处理后表达水平改变的基因共1 519个,其中上调的714个,下调的805个。选择与MF发生机制关系密切的趋化因子(C-C基元)配体7(CCL7)、基质金属蛋白酶26(MMP-26)及白细胞介素31受体α(IL31RA)等基因进行RT-PCR和Westernblot验证,结果与芯片检测一致。结论:利用基因芯片检测出ALD处理后FCFs多种基因表达发生改变,部分基因可能与MF有关。
AIM： To investigate differently expressed genes associated with cardiac fibrosis induced independently by aldosterone. METHODS： Fetal cardiac fibroblasts （FCFs） were isolated and cultured. Total RNA was extracted 8 hours after aldosterone administration. Then gene chips were used to screen these RNA samples, Some of candidate genes were confirmed by RT-PCR and Western blot. RESULTS： Differently expressed 1519 genes were screened. Up-regulated genes were 714 while downregulated genes were 805. The expression of CCL7, MMP-26 and IL31RA was tested by RT-PCR and western blot, the results is identical with those by gene chips. CONCLUSION： Gene chip can efficiently single out differently expressed genes induced dependently by aldosterone in FCFs. CCL7, MMP-26 and IL31RA may be associated with cardiac fibrosis induced by aldosterone.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2012年第4期350-353,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金面上项目(30871065)
关键词
醛固酮
心肌纤维化
基因芯片
aldosterone
myocardiac fibrosis
gene chip
