摘要
目的:建立同时测定火麻仁中的α-亚麻酸、亚油酸和油酸含量的HPLC方法。方法:色谱柱为Kromasil 100-5 C18(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸(80∶20)为流动相,流速1 mL.min-1,柱温30℃,检测波长203 nm。结果:α-亚麻酸在34.625～554 mg.L-1(r=0.999 9),亚油酸在56.375～902 mg.L-1(r=1),油酸在17.125～274 mg.L-1(r=0.999 9)呈良好的线性关系;平均回收率α-亚麻酸为96.38%,RSD 0.93%(n=6);亚油酸为97.79%,RSD 0.92%(n=6);油酸为97.06%,RSD 1.51%(n=6)。结论:本法简便准确,专属性强,重复性好,可作为火麻仁的质量控制的参考。
Objective：To establish a HPLC method for the determination of α-linoleic acid,linoleic acid and oleic acid in Hemp Seed.Method： The Kromasil 100-5 C18（4.6 mm×250 mm,5 μm） column was used with a mobile phase of acetonitrile-0.1% phosphoric acid（80∶20）,the flow rate was 1 mL·min-1,the column temperature was at 30 ℃,the detection wavelength was at 203 nm.Result： The linear ranges of α-linoleic acid,linoleic acid and oleic acid were 34.625-554 mg·L-1（r=0.999 9）,56.375-902 mg·L-1（r=1） and 17.125-274 mg·L-1（r=0.999 9） respectively.The average recoveries were 96.38% with RSD 0.93% for α-linoleic acid,97.79% with RSD 0.92% for linoleic acid,97.06% with RSD 1.51% for oleic acid.Conclusion： The method can be used to control the quality of α-linoleic acid,linoleic acid and oleic acid in Hemp Seed,which is simple,accurate,convenient,specific and repeatable.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第7期71-74,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技部973计划项目(2010CB735604)
