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大鼠局灶性脑缺血预处理后caspase-12mRNA及其蛋白的表达变化 被引量：2

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摘要 目的观察缺血预处理对大鼠脑缺血再灌注后caspase-12 mRNA及其蛋白表达和神经元凋亡的影响。方法 SD大鼠120只,随机分为假手术组(SO)、脑缺血再灌注组(MCAO)和脑缺血预处理组(BIP)3组,每组按照再缺血后12 h和1、2、3 d 4个时间点分为4个亚组。采用二次线栓法制备大鼠局灶性脑缺血预处理模型,用原位杂交法和Western blot法观察再缺血后各个时间点caspase-12 mRNA及其蛋白的表达变化,用流式细胞术检测神经细胞凋亡率。结果 (1)MCAO组12 h caspase-12 mRNA及其蛋白表达均达高峰,随再灌注时间延长其表达逐渐下降(P<0.05,P<0.01);BIP组较MCAO组caspase-12 mRNA表达明显降低,其蛋白表达也明显降低(P<0.05,P<0.01)。(2)MCAO组12 h细胞凋亡发生率显著增加,1 d时达到高峰,以后时间点逐渐下降(P<0.01);BIP组各个时间点神经元凋亡发生率较MCAO组显著降低(P<0.05,P<0.01)。结论脑缺血预处理可能通过抑制caspase-12表达发挥其神经保护作用。
作者 胡跃强 唐农 董少龙 毕方方 祝美珍 胡玉英 陈炜
机构地区 广西中医学院第一附属医院神经内科 广西中医学院科学实验中心 中南大学湘雅医院神经内科
出处 《广东医学》 CAS CSCD 北大核心 2012年第6期739-741,共3页 Guangdong Medical Journal
基金 国家自然科学基金资助项目(编号:30860354) 广西医疗卫生重点科研课题(编号:重200818)
关键词 缺血预处理 CASPASE-12 神经保护
分类号 R743.31 [医药卫生—神经病学与精神病学]
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参考文献8

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共引文献65

同被引文献22

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二级引证文献7

广东医学
2012年 第6期

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